EVALUATION OF MICRO LEAKAGE OF ROOT CANALS FILLED WITH DIFFERENT OBTURATION TECHNIQUES: AN IN VITRO STUDY

Authors

  • Maham Muneeb Lone Aga Khan University Hospital
  • Farhan Raza Khan Aga Khan University Hospital

Abstract

Background: Despite a plethora of studies done comparing different obturation techniques using gutta-percha, there is no consensus as to which obturation technique results in a ‘better’ sealing of root canal space. Aims of the study are to compare mean apical micro leakage in root canals of extracted teeth obturated with cold laterally compacted gutta-percha and thermoplasticised injectable gutta-percha using calcium hydroxide based sealer. Methods: It was an in-vitro experimental study carried out using extracted teeth. After access cavity preparation and canal preparation in 70 teeth, they were randomly divided into 2 groups and filled with two different obturation techniques using Sealapex sealer. Teeth were placed in 2.0% methylene blue solution, sectioned longitudinally, observed under microscope and images were captured using microscope attached camera. Amount of dye penetration was measured in millimetre from apex to most coronal part of dye penetration. Data was analysed using SPSS 20.0. Mean and standard deviation of continuous variables was computed. Independent Sample t- test was applied to compare micro leakage values in the two study groups. Level of significance was kept at 0.05. Results: According to the measurements, obturation with Obtura-II and Sealapex was leakier than the other group, with a mean dye penetration of 1.91±1.15mm. There was a statistically significant difference in dye penetration among the two groups. Conclusions: Cold lateral compaction plus Sealapex was the best combination for obturation as it exhibited least microleakage. Obtura II-Sealapex combination should be used with caution as it showed maximum microleakage. For obturation of single rooted teeth, we recommend cold lateral condensation with Sealapex sealer as it showed better sealability. Obtura-II and Sealapex should be used with caution in single rooted teeth as this group showed the maximum leakage.Keywords: Dye penetration; Endodontics; Obturation

References

Kakehashi S, Stanley HR, Fitzgerald RJ. The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats. Oral Surg Oral Med Oral Pathol 1965;20:340–9.

Molander A, Reit C, Dahlen G, Kvist T. Microbiological status of root‐filled teeth with apical periodontitis. Int Endod J 1998;31(1):1–7.

Farea M, Masudi S, Wan Bakar WZ. Apical microleakage evaluation of system B compared with cold lateral technique: In vitro study. Aust Endod J 2010;36(2):48–53.

WT Johnson JK. Obturation of the cleaned and shaped root canal system. In: HM Hargreaves SC, editor. Cohen’s Pathways of the Pulp. St. Louis: Mosby; 2011. p.349–88.

Leonard JE, Gutmann JL, Guo IY. Apical and coronal seal of roots obturated with a dentine bonding agent and resin. Int Endod J 1996;29(2):76–83.

Inan U, Aydemir H, Tasdemir T. Leakage evaluation of three different root canal obturation techniques using electrochemical evaluation and dye penetration evaluation methods. Aust Endod J 2007;33(1):18–22.

Rahimi S, Oskoee SS, Shahi S, Maljaei E, Abdolrahimi M, Mokhtari H, et al. In vitro comparison of apical microleakage following canal obturation with lateral and thermoplasticized gutta-percha compaction techniques. Afr J Biotechnol 2010;9(48):8235–40.

Dultra F, Barroso JM, Carrasco LD, Capelli A, Guerisoli DMZ, Pécora JD. Evaluation of apical microleakage of teeth sealed with four different root canal sealers. J Appl Oral Sci 2006;14(5):341–5.

Carrotte P. Endodontics: part 8. Filling the root canal system. Br Dent J 2004;197(11):667–72.

Almeida JF, Gomes BP, Ferraz CC, Souza-Filho FJ, Zaia AA. Filling of artificial lateral canals and microleakage and flow of five endodontic sealers. Int Endod J 2007;40(9):692–9.

WU MK, De Gee AJ, Wesselink PR, Moorer WR. Fluid transport and bacterial penetration along root canal fillings. Int Endod J 1993;26(4):203–8.

Limkangwalmongkol S, Burtscher P, Abbott PV, Sandler AB, Bishop BM. A comparative study of the apical leakage of four root canal sealers and laterally condensed gutta-percha. J Endod 1991;17(10):495–9.

Punia SK, Nadig P, Punia V. An in vitro assessment of apical microleakage in root canals obturated with gutta-flow, resilon, thermafil and lateral condensation: A stereomicroscopic study. J Conserv Dent 2011;14(2):173–7.

Bodrumlu E, Tunga U. Apical leakage of Resilon obturation material. J Contemp Dent Pract 2006;7(4):45–52.

Sadr S, Golmoradizadeh A, Raoof M, Tabanfar MJ. Microleakage of Single-Cone Gutta-Percha Obturation Technique in Combination with Different Types of Sealers. Iran Endod J 2015;10(3):199–203.

Elias I, Guimarães GO, Caldeira CL, Gavini G, Cai S, Akisue E. Apical sealing ability comparison between GuttaFlow and AH Plus: in vitro bacterial and dye leakage. J Health Sci Inst 2010;28(1):77–9.

Sevimay S, Kalayci A. Evaluation of apical sealing ability and adaptation to dentine of two resin‐based sealers. J Oral Rehabil 2005;32(2):105–10.

Souza EM, Pappen FG, Shemesh H, Bonanato-Estrela C, Bonetti-Filho I. Reliability of assessing dye penetration along root canal fillings using methylene blue. Aust Endod J 2009;35(3):158–63.

Al-Dewani N, Hayes SJ, Dummer PMH. Comparison of laterally condensed and low-temperature thermoplasticized gutta-percha root fillings. J Endod 2000;26(12):733–8.

Schirrmeister J, Kielbassa A. Apikale Dichtigkeit von Wurzelkanalsealern auf Calciumphosphat-Basis im Vergleich zu marktüblichen Füllpasten. Dtsch Zahnärztliche Z 2005;60(8):446–56.

Chawla A, Kapur R, Singh P. Assessment of apical seal obtained by three obturating techniques using dye penetration method and macro lens photography: an in-vitro study. Endodontology 2012;24:29–35.

Tomson R, Polycarpou N, Tomson P. Contemporary obturation of the root canal system. Br Dent J 2014;216(6):315–22.

Goodman A, Schilder H, Aldrich W. The thermomechanical properties of gutta-percha. II. The history and molecular chemistry of gutta-percha. Oral Surg Oral Med Oral Pathol 1974;37(6):954–61.

Guigand M, Glez D, Sibayan E, Cathelineau G, Vulcain JM. Comparative study of two canal obturation techniques by image analysis and EDS microanalysis. Br Dent J 2005;198(11):707–11.

Schilder H, Goodman A, Aldrich W. The thermomechanical properties of gutta-percha. Part V. Volume changes in bulk gutta-percha as a function of temperature and its relationship to molecular phase transformation. Oral Surg Oral Med Oral Pathol 1985;59(3):285–96.

Varun K, Harpreet S, Rajinder B, Samrity P. Qualitative and Quantitative Comparative Evaluation of Sealing Ability of Guttaflow, Thermoplasticized Gutta Percha and Lateral Compaction for Root Canal Obturation: A Cohort, Controlled, Ex-Vivo Study. Oral Health Dent Manag 2013;12(3):155–61.

Gutmann JL, Rakusin H. Perspectives on root canal obturation with thermoplasticized injectable gutta‐percha. Int Endod J 1987;20(6):261–70.

Peng L, Ye L, Tan H, Zhou X. Outcome of root canal obturation by warm gutta-percha versus cold lateral condensation: a meta-analysis. J Endod 2007;33(2):106–9.

Published

2018-02-13

Issue

Vol. 30 No. 1 (2018): JOURNAL OF AYUB MEDICAL COLLEGE, ABBOTTABAD

Section

ORIGINAL ARTICLE

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