EFFECT OF MONOSODIUM GLUTAMATE ON THE SERUM ESTROGEN AND PROGESTERONE LEVELS IN FEMALE RAT AND PREVENTION OF THIS EFFECT WITH DILTIAZEM

Muhammad Sarwar Zia, Khadija Qamar, Ruhila Hanif, Moazzam Khalil

Abstract


Background: Glutamate is found in a wide variety of foods. It  induces the uterine fibroid in the rats by increasing the levels of estradiol. Diltiazem is an effective preventive medication. This study was conducted to analyse the effect of monosodium glutamate on the serum estrogen and progesterone levels in adult Sprague Dawley rat and its prevention with diltiazem. Methods: This Laboratory based randomized controlled trial was conducted in the department of Anatomy, Army Medical College, Rawalpindi in collaboration with National Institute of Health (NIH), Islamabad from 9th April to 23rd April 2012. In this experimental study, 30 adult female Sprague Dawley rats of average weight of 500 g were randomly assigned into three groups. The experimental group B was given 0.08 mg/kg of monosodium glutamate (MSG) orally and experimental group C was given 0.08 mg/kg of MSG and 10 mg/kg of diltiazem in distilled water orally for 14 days. The control group (A) received only laboratory diet. Using intracardiac route 5 ml blood was taken from each animal for hormonal assay. Results: Hormone assay of the serum in the experimental group B showed increase in serum estrogen and progesterone levels as compared to the group A and there was minor increase in the hormonal levels in group C. Conclusion: MSG causes increase in the serum estrogen and progesterone levels in adult female rats and diltiazem prevents this effect.

Keywords: Diltiazem, Estrogen, Monosodium Glutamate, Progesterone

Full Text:

PDF

References


Rogers PP, Blundell JE. Umani and appetite: Effects of monosodium glutamate on hunger and food intake in human subjects. Physiol Behav 1990;48(6):801–4.

Iwase M, Yamamoto M, Iino K, Ichikawa K, Shinohara N, Yoshinari F. Obesity induced by neonatal monosodium glutamate treatment in spontaneously hypertensive rats: an animal model of multiple risk factors. Hypertens Res 1998;21:1–6.

Gobatto CA, Mello MA, Souza CT, Ribeiro IA. The monosodium glutamate (MSG) obese rat as a model for the study of exercise in obesity. Res Commun Mol Pathol Pharmacol 2002;111(1–4):89–101.

Belluardo N, Mudo G, Bindoni M. Effect of early destruction of the mouse arcuate nucleus by MSG on age dependent natural killer activity. Brain Res 1990;534:225–333.

Nayanatara AK1, Vin- odini NA1, Damodar G1, Ahemed B1, Rameshwamy CR1, Shabarianth. Role of ascorbic acid in monosodium glutamate mediated effect on testicular weight, sperm morphology and sperm count, in rat testis. J Chin Clin Med 2008;3(1):1–5.

Eweka AO, Eweka A, Om’Iniabohs FAE. Histological studies of the effects of monosodium glutamate on the fallopian tube of adult female Wistar rats. N Am J Med Sci 2010;2(3):146–9.

Obochi GO, Malu SP, Obi-Abang M, Alozie Y, Iyam M. Effect of garlic extracts on MSG induced fibroid in wistar rats. Pak J Nutr 2009;8:970–6.

Class SA, Glasser SP. Long-acting diltiazem HCL for chronotherapeutic treatment of hypertension and chronic stable angina pectoris. Expert Opin Pharmacother 2005;6:765–76.

Gabrielli A, Gallagher TJ, Caruso LJ, Bennet NT, Layon JA. Diltiazem to treat sinus tachycardia in critically ill patients: A four year experience. Ctitical Care Med 2001;29:1874–9.

Grossman E, Messerli FH. Calcium antagonists. Prog Cardiovasc Dis 2004;47(1):34–57.

Bojanic VV, Bojanic ZZ, Najman S, Savic T, Zivanov-Curlis J, Tomin J. Diltiazem prevention of monosodium glutamate toxicity on hypothalamus in Wistar rats. Arch Oncol 2004;12(suppl 1):19–20.

Lamperti, Blaha. Ovarian Function in the Hamsters Treated with Monosodium Glutamate. Biol Reprod 1979;21:923–8.


Refbacks

  • There are currently no refbacks.


Contact Number: +92-992-382571

email: [jamc] [@] [ayubmed.edu.pk]