DERMATOPHYTE GROWTH AND DEGRADATION OF HUMAN STRATUM CORNEUM IN VITRO (PATHOGENESIS OF DERMATOPHYTOSIS).

Azam Jah Samdani

Abstract


Background: This study was carried out to determine growth of dermatophytes using human
stratum corneum in vitro and the degrading effect of Keratinases (Proteinases) on stratum corneum
for a complete understanding of the host parasite relationship. Method: Trichophyton rubrum
isolates derived from patients with tinea cruris infections were obtained from the Department of
Medical Microbiology, University Hospital of Wales, U.K. Human stratum corneum sterilized
with ethylene oxide was used as a nitrogen source in agar culture medium plates. Result: Fungal
growth took place in plates which contained human stratum corneum particles while there was no
growth in the plates without stratum corneum at three weeks after initiation. There was a gradual
disappearance of the particles of stratum corneum from the plates at the end of the third week
Conclusion: The growth of organisms in plates with human stratum corneum and their
disappearance at third week suggested that stratum corneum was not only source of nutrition for
the dermatophytes, but also the growing fungal mycelia and the proteinases induced by them were
playing a part in the digestion of granules and thus may have an important role in the pathogenesis
of dermatophyte infections.
Key-words: Proteinases, Stratum -corneum, Dermatophytes

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