COMPARATIVE CELLULAR IMMUNE HOST RESPONSE IN ACUTE VS HEALED LESIONS OF CUTANEOUS LEISHMANIASIS

Simeen Ber Rahman, Arfan ul Bari

Abstract


Background: Cutaneous leishmaniasis (CL) has become a major public health problem in
Pakistan and research is now focused to characterize the host’s immune response, its clinical
correlation and subsequent implications in management in this disease. This study was done to
evaluate cellular immune host response in patients with active and healed CL and its possible
implications in prevention of disease in susceptibles. Methods: This cross sectional and
comparative study was conducted in Armed Forces Institute of Pathology (AFIP) and Military
Hospital (MH) Rawalpindi (1998-2000). 30 biopsies of active skin lesions and 15 biopsies from
healed lesions, after processing, were studied for various immunophenotype cells by using
monoclonal antibodies. Total and differential T cell counts were recorded in these skin tissues.
Non parametric Kruskal-Wallis Test for one way ANOVA was used to compare the median cell
counts between active, healed and normal skin and p-value <0.05 was considered significant.
Results: The total cell counts, CD3+ cells and CD57+ (NK) cells were found statistically different
(p=<0.001) when active forms of the disease were compared with healed lesions and normal skin
tissue. The difference was not significant (p=>0.05) on comparing healed lesions with normal skin
tissue biopsies except in case of CD3+ cell counts (p=<0.05). However, CD4+, CD8+ and
CD19+(Plasma cells) counts were never seen significant (p=>0.05) on comparison. Conclusion:
NK cells and gamma delta cells seem to be responsible for limitation of the disease and
elimination of the parasite from the lesion in cases of acute cutaneous leishmaniasis.
Keywords: Leishmaniasis, Cutaneous leishmaniasis, Cellular immune host response

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