DEVELOPMENT OF ENZYME IMMUNOASSAY AND ITS ROLE IN REPRODUCTIVE ENDOCRINOLOGY

Mir Hassan Khan, M. Subhan Qureshi

Abstract


Radioimmunoassay is a powerful technique developed in 1960 by Solomon Berson and
Rosalyn Yallow, who first devised a R1A for the measurement of insulin concentration in plasma1.
The technique was far more sensitive and specific than any method existed at the time. RIA is used
to analyse many biological substances that are present in minute amounts, including steroids, cyclic
nucleotides, peptide hormones, viruses, bacterial antigen, enzymes, prostaglandins and drugs.
Inspite of certain advantages of RIA in terms of sensitivity, specificity and wide
applicability2, it has a number of disadvantages including high capital cost, as the devises used are
very expensive and their maintenance in developing countries is extremely difficult task. Beside
radiation hazards are a constant threat to personnel working in RIA laboratory. Decomposition of
radiolabeled proteins upon storage is also a serious problem in RIA, affecting the sensitivity and
reproducibility of assay system.

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