ANTI-HCV ANTIBODIES DETECTION-A COMPARISON BETWEEN METHODOLOGY: ELISA VS DIPSTICK ASSAY

Shahid Mahmood Sheikh, Muhammad Tayyab, Ghazala Jaffary, Tahira Tasneem, Abdul Sattar, Iftikhar Ali

Abstract


Hepatitis C accounts for 70-95% cases of post-transfusion hepatitis in different parts of the world. The high prevalence
of antibodies to hepatitis C virus (HCV) among frequently blood transfused patients (e.g. thalassaemic), those who
receive blood products (e.g. haemophiliacs), and among intravenous drug abusers confirmed the parenteral route as
the major route of HCV infection. To promote screening of blood donors for HCV antibodies, a Dipstick HCV assay
was compared with third generation enzyme immunoassay. Fifty diagnosed cases of hepatitis C and twenty healthy
control subjects were analyzed by both methods and hundred per cent same results were found.

Full Text:

PDF

References


Shah SHA. Abid S. Khan All. Chronic non-B,

non-C Hepatitis Report from Pakistan Am J

Gastroenterol 1992; 87:1061-62.

Wyngaarden .113. Smith LH. Bennett JC (eds).

Cecil Textbook of Medicine. 19lh lid

Philadelphia: VVB Saunders Company. 1992:

-78.

Ahmed M. Qureshi MS. Mushtaq S. Khan FA,

Ahmed SA. Zaheeruddin Acute sporadic hepatitis

non-A. non-B: Clinical features and biochemical

profile. JPMA 1989; 307-399.

Wang JEH A study on the epidemiology of

hepatitis C infection among blood donors in

Singapore. .1 Public Health Med 1995; 17: 387-

Bradley D. McCaustland K. Krawcznski K.

Spelbring .1. Humphrey C. Cook EH. Hepatitis C

virus: Buoyant density of the factor VIII derived

isolated in sucrose. .1 Med Virol 1991.34:206-8.

Choo Ql. Richman K. Han. JH. el al. Genetic

organization and diversity of the hepatitis C virus.

Proc Natl Acad Sci USA 1991:88:2451-55.

Malik IA. Ahmad N, Luqman M, el al. Hepatitis C

as a cause of chronic liver disease in northern

Pakistan. JPMA 1992: 67-68.

Weiner AJ. Kuo G. Bradley DW. et al. Detection

of hepatitis C viral sequences in non-A. non-B

hepatitis. Lancet 1990:335 1-3.

Malik IA. The prevalence, pattern and prevention

of viral hepatitis in Pakistan, souvenir 21s1

National PAP Conference, 1997: 66-67

Ramia S Hepatitis C virus: Molecular virology and

its implications for serologic diagnosis. Saudi .1

kidney Dis Transplant 1995:6(2) 190-96.

Al-Faleh I Z. Ramia S. Hepatitis C virus (HCV)

infection in Saudi Arabia: A review. Ann Saudi

Med 1997; 17:77- 82.

Van-del-Poel CL. Reesink II. Schaaberg W. el al.

infectivity of blood seropositive for hepatitis C

virus antibodies. Lancet 1990: 335:558-60.

Tremolada f. Casarim C, tagger A, et al. Antibody

to hepatitis C virus in post-transfusion hepatitis.

Ann Intern Med 1991; 114: 277-81

Fusconi M. I.enzi M. Ballardini G. et al Anti-I ICV

testing in autoimmune hepatitis and primary

biliary eirrhosisl. Lancet 1990: 336 823.

Mattsson I. Gutierrez R. Dawson GIL Lesniewski

RR, Mushahawar LK, Weiland O. antibodies to

recombinant and synthetic peptides derived from

the hepatitis C virus genome in long-term studied

patients with post-transfusion hepatitis C. Scand. J

Gastroenterol 1991:26: 1257-62.

Craxi A, Valenza M, Fabiano c. et al. Thirdgeneration hepatitis C virus tests in asymptomatic

anti-IIC’V positive blood donors. .1 Hepatol 1994;

: 730-34.

Zauer HI.. Virelind H. Van Exel-Oehlers P.I. et al.

Confirmation of hepatitis C infection: A

comparison of five immunoblot assays,

Transfusion 1994; 34: 603-607.

Okamoto H. Tsuda f. Machida a. et al. Antibodies

against synthetic oliopeptides deduced from the

putative core gene for the diagnosis of hepatitis C

virus Hepatology 1992: 15: 180-86"

Nagavama R. Miyaakae K. Tsuda f. Okamoto H

IgM antibody to a hepatitis C virus core peptide

(CPU) for monitoring activity of liver disease in

patients with acute or chronic hepatitis C. J Med

Virol 1994; 42: 311-17.

Issar SK, rantakrishna BS, Ramakrishna B,

Christopher S. Samuel BU. Jhon T.I. Prevalence

and presentation of hepatitis C related chronic

liver disease in Southern India .1 Trap Med

Hygiene 1995: 98: 161-65.

Shimizu YK. Weiner AJ. Rosenblatt .1. et al. Early

events in hepatitis C’ virus infection of

chimpanzees. Proc Natl Acad Sei USA 1990; 87:

-44


Refbacks

  • There are currently no refbacks.


Contact Number: +92-992-382571

email: [jamc] [@] [ayubmed.edu.pk]