ANTI-INFECTIVE POTENTIAL OF A MEDICINAL PLANT PIMPENELLA STEWARTII USED TRADITIONALLY IN HAZARA
AbstractBackground: Medicinal plants have been used since long as folk medicines and also in Ayuvedic and Unani systems of treatment. They have importance to save human and animal lives because of their utilization in the herbal medicines. In all over the word plants are in constant use for curing of various kinds of diseases. The herbal medicines are effective due to presence of bioactive compounds along with vitamins and minerals in them. Moreover, plants produce photo-chemicals mainly secondary metabolites which have significant pharmaceutical activities like antioxidant, antimicrobial, pesticidal and anti-tumour activity. They are very effective and have little or no side effects. Methods: Five microbial strains including three fungal and two bacterial species were tested for the efficacy of plant materials by Agar diffusion method. The fungal species were Aspergilus flavus, Aspergilus niger and Alternaria alternata while bacterial species included Arvenia caratovora and Xanthomans spp. Results: Results obtained for methanolic extracts of Pimpenella stewartii against Aspergilus flavus, Aspergilus niger and Alternaria alternata showed significant antifungal activity. The antibacterial screening of Methanolic extracts of Pimpenella stewartii were evaluated against Arvenia caratovora and Xanthomans spp. The best zone of inhibition was found against Xanthomans spp (97.33±2 mm) at 250 ppm concentration followed by Arvenia caratovora (49.7±14 mm) at same concentration.Keywords: Pimpenella stewartii, Aspergillus niger, Xanthomans spp, Methanolic extract
1. Kiringe JW. A survey of traditional health remedies used by the Maasai of Southern Kaijiado District, Kenya. 2006;4:61–73.
Egwaikhide P, Gimba C. Analysis of the Phytochemical Content and Anti-microbial Activity of Plectranthus glandulosis whole plant. Middle-East J Sci Res 2007;2(3-4):135–8.
Jagessar RC, Mohamed A, Gomes G. Antibacterial and antifungal activity of leaf extracts of Luffa operculata, vs. Peltophorum Pterocarpum, against Candida albicans, Staphylococcus aureus and Escherichia coli. Nat Sci 2007;5(4):81–93.
Ceylan E, Fung DYC. Antimicrobial activity of spices. J Rapid Methods Autom Microbiol 2004;12(1):1–55.
Shakeri A, Hazeri N, Vlizadeh J, Ghasemi A, Tavallaei FZ. Phytochemical screening, Antimicrobial and antioxidant activities of Anabasis aphylla L. extracts. Kragujev J Sci 2012;34(34):71–8.
Nascimento GG, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Braz J Microbiol 2000;31(4):247–56.
Elisabetsky E, Amodor TA, Albuquerque RR, Nunes DS, Carvalho Ado C. Analgesic activity of Psychotria colorata (Wild. ex R&S). Muell. Arg. alkaloids. J Ethnopharmacol 1995;48(2):77–83.
Hussain MA, Gorsi MS. Antimicrobial activity of Nerium oleander Linn. Asian J Plant Sci 2004;3(2):177–80.
Nitta T, Arai T, Takamatsu H, Inatomi Y, Murata H, Iinuma M, et al. Antibacterial activity of extracts prepared from tropical and subtropical plants on methicillin-resistant Staphylococcus aureus. J Health Sci 2002;48(3):273–6.
Costa ES, Hiruma-Lima CA, Lima EO, Sucupira GC, Bertolin AO, Lolis SF, et al. Antimicrobial activity of some medicinal plants of the Cerrado, Brazil. Phytother Res 2008;22(5):705–7.
Ndhlala A, Stafford G, Finnie J, Van Staden J. In vitro pharmacological effects of manufactured herbal concoctions used in KwaZulu-Natal South Africa. J Ethnopharmacol 2009;122(1):117–22.
Afzal S, Afzal N, Awan MR, Khan TS, Khanum AGR, Tariq S. Ethno-botanical studies from Northern Pakistan. J Ayub Med Coll Abbottabad 2009;21(1):52–7.
Gülçın İ, Oktay M, Kıreçcı E, Küfrevıoǧlu Öİ. Screening of antioxidant and antimicrobial activities of anise (Pimpinella anisum L.) seed extracts. Food Chem 2003;83(3):371–82.
Khalid A, Rehman U, Sethi A, Khilji S, Fatima U, Khan MI, et al. Antimicrobial activity analysis of extracts of Acacia modesta, Artimisia absinthium, Nigella sativa and Saussurea lappa against Gram positive and Gram negative microorganisms. Afr J Biotechnol 2011;10(22):4574–80.
Olukoya D, Odugbemi T, Bamgbose S. Some aspects of traditional therapy of Gonorrhoea in Lagos, Nigeria. J Res Ethno-Med 1986;1:26–9.
Adesanya SA. In: Theories and realities. Inaugural lecture series 181, Published by Obafemi Awolowo University Press Ltd Ile-Ife, Osun State Nigeria, 2005.
Eloff J. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol 1998;60(1):1–8.
Akhtar A, Deshmukh A, Bhonsle A, Kshirsagar P, Kolekar M. In vitro Antibacterial activity of Pimpinella anisum fruit extracts against some pathogenic bacteria. Vet World 2008;1(9):272–4.