EFFECT OF ACACIA NILOTICA LEAVES EXTRACT ON HYPERGLYCAEMIA, LIPID PROFILE AND PLATELET AGGREGATION IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Munazza Asad, Muhammad Aslam, Tahir Ahmad Munir, Amina Nadeem

Abstract


Background: To consider new hypoglycaemic, anti-hyperlipidaemic and anti-platelet aggregation
sources, aqueous methanol extract of Acacia Nilotica (AN) leaves was investigated in streptozotocin
induced diabetic rats. Methods: Diabetes mellitus was induced in 90 out of 120 male albino rats by
administering 50 mg/Kg bodyweight (bw) streptozotocin intraperitonealy, and was confirmed by
measuring fasting blood glucose level >200 mg/dL on 4th post-induction day. The rats were equally
divided into 4 groups, A (normal control), B (diabetic control), C (diabetics rats treated with plant
extract) and group D (diabetics rats treated with glyburide). The rats of group C and D were given
single dose of 300 mg/Kg bw, AN extract, and 900 µg/Kg bw glyburide respectively for 3 weeks.
Blood glucose levels were measured by glucometer, platelet aggregation by DiaMed method, β-
thromboglobulin and insulin by ELISA technique, and lipid components were measured by enzymatic
calorimetric method. Results: Significant differences (p<0.05) were noticed in blood glucose, serum
insulin, platelet aggregation and triglyceride levels in diabetic rats treated with AN extract and
glyburide as compared to diabetic controlled rats. A significant difference (p<0.05) in β-
thromboglobulin and LDL levels was also noticed in rats treated with glyburide than the diabetic
controlled rats. The levels of fasting blood glucose, β-thromboglobulin and platelet aggregation were
significantly reduced (p<0.05) in diabetic rats treated with glyburide than AN extract treated rats.
Conclusions: Administration of AN leaves extract showed hypoglycaemic and anti-platelet
aggregation activity in diabetic rats as that of glyburide.
Keywords: Acacia Nilotica, Hypoglycemia, Streptozotocin

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References


Alvin CP. Diabetes Mellitus. In: Fauci AS, Braunwald E, Kasper

DL, Hauser SL, Longo DL, Jameson JL, Loscalzo J. Harrison’s

Principles of Internal Medicine. 17th ed. New York: McGraw Hill

; p. 2275–304.

Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence

of diabetes, estimates for the year 2000 and projections for 2030.

Diabetes Care 2004;27:1047–53.

Hink U, Li H, Mollnau H, Oelze M, Matheis E, Hartmann M, et

al. Mechanisms underlying endothelial dysfunction in diabetes

mellitus. Circ Res 2001;88:14–22.

Cosentino F, Eto M, Paolis PD, Loo B, Bachschmid M, Ullrich

V, et al. High glucose causes upregulation of cyclooxygenase 2

and alters prostanoid profile in human endothelial cells, Role of

protein kinase C and reactive oxygen species. Circulation

;107:1017–23.

Halliwell B, Gutteridge JMC. Lipid peroxidation oxygen radicals,

cell damage, and antioxidant therapy. Lancet 1994;1:1396–7.

WHO Expert Committee on Diabetes Mellitus Second Report

Series 646, Geneva; 1981;p. 61.

J Ayub Med Coll Abbottabad 2011;23(2)

http://www.ayubmed.edu.pk/JAMC/23-2/Munazza.pdf 7

Roman-Ramos R, Flores-Saenz JL, Alarcon-Aguilar FJ. Antihyperglycemic effect of some edible plants. J Ethnopharmacol

;48:25–32.

Sundaram R, Mitra SK. Antioxidant activity of ethyl acetate

soluble fraction of Acacia Arabica barks in rats. Indian J

Pharmacol 2007;39:33–8.

Jones J, Sharma A, Hasen KW, Iikova H, Patane G, Laybutt R, et

al. Chronic hyperglycemia triggers loss of pancreatic beta cell

differentiation in an animal model of diabetes. J Biol Chem

;274:14112–21.

Paul W, Queen LR, Page CP, Ferro A. Increased platelet

aggregation in vivo in the Zucker diabetic fatty rat: differences

from the streptozotocin diabetic rat. Br J Pharmacol

;150:105–11.

Ganda OP, Rossi AA, Like AA. Studies on streptozotocin

diabetes. Diabetes 1976;25:595–603.

Natarajan A, Zaman AG, Marshall SM. Platelet hyperactivity in

type 2 diabetes: role of anti-platelet agents. Diabetes Vasc Dis

Res 2008;5:138–44.

Takasu N, Komiya I, Asawa T, Nagasawa Y, Yamada T.

Streptozotocin and alloxan induced H2O2 generation and DNA

fragmentation in pancreatic islets. Diabetes 1991;40:1141–5.

Nukatsuka M, Yoshimura Y, Nishida M, Kawada J. Importance

of the concentration of ATP in rat pancreatic beta cells in the

mechanism of streptozotocin-induced cytotoxicity. J Endocrinol

;127:161–5.

Maqsood A, Fatima Z, Tanveer S, Zabta M. Anti-diabetic and

hypolipidemic effects of aqueous methanolic extract of acacia

nilotica pods in alloxen induced diabetic rats. Scand J Lab Anim

;35:29–34.

Nukatsuka M, Yoshimura Y, Nishida M, Kawada J. Importance

of the concentration of ATP in rat pancreatic beta cells in the

mechanism of streptozotocin-induced cytotoxicity. J Endocrinol

;127:161–5.

Scheen AJ, Lefebvre PJ. Oral anti-diabetic agents: A guide to

selection. Drugs 1998;55:225–36.

Kumar R. Chemical and biochemical nature of fodder tree

tannins. J Agriculture Food Chem 1983;31:1346–66.

Hirosh T, Mitsuy I, Mi T, Jin Ew, Tosiyasu S, Ikuko K. Effect of

green tea on blood glucose levels and serum proteomics patterns

in diabetic mice and on glucose metabolism in healthy humans.

BMC Pharma 2004;4:18–30.

Liu X, Kim JK, Li Y, Li J, Liu F, Chen X. Tannic acid stimulates

glucose transport and inhibits adipocyte differentiation in 3T3-L1

cells. Am J Clin Nutr 2005;135:165–71.

Tiwari AK, Madhusudana RJ. Diabetes mellitus and multiple

therapeutic approaches of Phyto-chemicals: present status and

future prospects. Curr Sci 2002;83:30–8.

Wadood A, Wadood N, Shah SA. Effects of Acacia Arabica and

Caralluma edulis on blood glucose levels of normal and alloxan

diabetic rabbits. J Pak Med Assoc 1989;39:208–12.

Pande MB, Talpada PM, Patel ZN, Purohit LP, Shukla PC. Note

on processed babul feeding to mature Kankrej bullocks. Indian J

Animal Sci 1980;52:798–9.

Gilani AH, Molla N, Atta-ur-Rehman, Shah BH. Role of natural

products in modern medicine. J Pharm Med 1992;2:111–8.

Rasheed H, Tirmizi AH, Salahuddin F, Rizvi NB, Arshad M,

Farooq SZ, et al. Calcium signaling in human platelet

aggregation mediated by platelet activating factor and calcium

ionophre, A23187. J Biol Sci 2004;4:117–21.

Shah BH, Milligan G. The gonadotrophic releasing hormone

receptor of αT3-1 cells regulates cellular levels of both of the

phosphoionositidase C-linked G proteins, Gqα and G11α,

equally. Mol Pharmac 1994;46:1–7.

Bretschneider E, Glusa E, Schror K. ADP, PAF, and adrenalineinduced platelet aggregation and thromboxane formation are not

affected by a thromboxane receptor antagonist at physiological

external Ca+2 concentrations. Thromb Res 1994;5:233–42.

Fritschi J, Christe M, Lammle B, Marbet GA, Berger W, Duckert

F. Platelet aggregation, beta-thromboglobulin and platelet factor

in diabetes mellitus and in patients with vasculopathy. Thromb

Haemost 1984;52:236–9.

Yamada K, Mori K, Goto Y. Platelet aggregation and plasma

levels of beta-thromboglobulin in diabetes mellitus. Tohoku J

Exp Med 1981;135:423–9.

Taomoto K, Asada M, Kanazawa Y, Matsumoto S. Usefulness of

the measurement of plasma beta-thromboglobulin (beta-TG) in

cerebrovascular disease. Stroke 1983;14:518–24.

Socher M, Baquer NZ, Mclean P. Glucose under utilization in

diabetes: comparative studies on the changes in the activities of

enzymes of glucose metabolism in the rat kidney and liver. Mol

Physiol 1985;7:51–68.

Arkkila PE, Koskinen PJ, Kantola IM, Ronnemma T, Seppanen

E, Vikari JS. Diabetic complications are associated with liver

enzyme activities in people with type 1 diabetes mellitus.

Diabetes Res Clin Pract 2001;52:113–8.

Lee KT, Shon IC, Kim DH, Choi JW, Kwon SH, Park HJ.

Hypoglycemic and hypolipidemic effect of tectorigenin and

kaikasaponin III in streptozotocin induced diabetic rats and their

antioxidant activity in-vitro. Arch Pharm Res 2000;23:461–6.

Maciejewski R, Rusinski P, Burski K, Figura T. Changes in

glucose, cholesterol and serum lipid function levels in

experimental diabetes. Ann Univ Mariae Curie Sklodowska

;56:363–8.

Bopanna KN, Kannan J, Sushma G, Balaraman R, Rathod SP.

Antidiabetic and antihyperlipidemic effect of neem seed, kernel

powder on alloxen diabetic rabbits. Ind J Pharmacol

;29:162–7.

Rajkumar L, Govidarajulu P. Increased degradation of dermal

collagen in diabetic rats. Ind J Exp Biol 1991;29:1081–3.

Kim JS, Jung BJ, Choi CW, Kim SC. Hypoglycemic and

antihyperlipidemic effect of four Korean Medicinal plants in

alloxen induced diabetic rats. Am J Biochem Biotechnol

;2:154–60.


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