ANTI-ARTHRITIC EFFECT OF THYMOQUINONE IN COMPARISON WITH METHOTREXATE ON PRISTANE INDUCED ARTHRITIS IN FEMALE SPRAGUE DAWLEY RATS
Abstract
Background: Rheumatoid arthritis inflammatory joint disease which is chronic in nature. Since long various researches are carried out to find the remedy of this disease but still a lot of work needed to be done. Methods: This comparative study was performed from March to August 2013 at Postgraduate Medical Institute, Lahore. A total of thirty-two female Sprague-Dawley rats were randomly divided into four equal groups (n=8); group A was kept as healthy control, group B was kept as positive control, group C was treated with thymoquinone and group D was treated with methotrexate. Arthritis developed within two weeks in group B, group C and group D after giving a single shot of pristane intradermally. Treatment was started on day 15. At day 30 (time of dissection) paw weight and histopathological sections of ankle joints of all the animals were taken. Results: The results shown significant rise in paw weight and score of histopathological parameters in group B, group C and group D when compared with healthy control rats. Thymoquinone and methotrexate treated groups shown reduction in paw weight and score of histopathological parameters when compared to positive control rats with p-value 0.001each. The difference between groups C and D was insignificant (p-value 0.062). Conclusion: Study results supported the anti-inflammatory and disease modifying activities of thymoquinone as it significantly reduces both paw weight and histological parameters of joint inflammation.Keywords: Thymoquinone; Pristane; Paw weight; Joint histopathology; Rheumatoid arthritis; MethotrexateReferences
Anish R. Rheumatoid Arthritis of the Foot and Ankle [Internet]. Orhtoinfo. American Academy of Orthopaedic Surgeons [cited 2017 Feb 12]. Available from: https://orthoinfo.aaos.org/en/diseases--conditions/rheumatoid-arthritis-of-the-foot-and-ankle
Gordon A. Rheumatoid arthritis: MedlinePlus Medical Encyclopedia [Internet]. [cited 2017 Fed 12]. Available from: https://medlineplus.gov/ency/article/000431.htm
Rheumatoid Arthritis of the Foot and Ankle. [Internet]. American Orthopaedic Foot & Ankle Society. [cited 2017 Feb 12]. Available from: http://www.aofas.org/footcaremd/conditions/ailments-of-the-midfoot/Pages/Rheumatoid-Arthritis.aspx
Anonymous. Rheumatoid Arthritis in the Foot and Ankle. What is rheumatoid arthritis. [Internet]. Foot Health Facts, American College of Foot and Ankle Surgeons. [cited 2017 Feb 12]. Available from: https://www.foothealthfacts.org/conditions/rheumatoid-arthritis-in-the-foot-and-ankle
Danish I, editor. Medical Diagnosis and Management. 8th ed. Karachi: Johar Publications; 2010.
Gillani AU, Jabeen Q, Khan MA. A review of medicinal uses and pharmacological activities of Nigella sativa. Pak J Biol Sci 2004;7(4):441–51.
Ragheb A, Attia A, Eldin WS, Elbarbry F, Gazarin S, Shoker A. The protective effect of thymoquinone, an anti-oxidant and anti-inflammatory agent, against renal injury: a review. Saudi J Kidney Dis Transpl 2009;20:741–52.
Chaieb K, Kouidhi B, Jrah H, MahdouaniK,Bakhrouf A. Antibacterial activity of thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation. BMC Complement Altern Med 2011;11:29.
El-Mezayen R, El-Gazzar M, Nicolls MR, Marecki JC, DreskinSC, Nomiyama H. Effect of thymoquinone on cyclooxygenase expression and prostaglandin production in a mouse model of allergic airway inflammation. Immunol Lett 2006;106(1):72–81.
Hosseinzadeh H, Eskandariz M, Ziaeez T. Antitussive effect of thymoquinone, a constituent of Nigella Sativa seeds, in guinea pigs. Pharmacologyonline 2008;2:480–4.
Amira E, Abdel A, Nesrine S, El-Sayed, Laila G, Mahran. Anti-asthmatic and anti-allergic effects of thymoquinone on airway-induced hypersensitivity in experimental animals. Pak J Pharm Sci 2011;1:109–17.
Pari L, Sankaranarayanan C. Beneficial effects of thymoquinone on hepatic key enzymes in streptozotocin–nicotinamide induced diabetic rats. Life Sci 2009;85(23-26):830–34.
Faisal R, Chiragh S, Popalzai AJ, Khalil Ur Rehman. Anti-inflammatory effect of thymoquinone in comparison with methotrexate on pristane induced arthritis in rats. J Pak Med Assoc 2015;65(5):519–25.
Faisal R, Imran U. Comparative evaluation of thymoquinone and methotrexate in lung inflammation in murine model of rheumatiod arthritis. J Postgrad Med Inst 2015;29(2):88–92.
Faisal R, Gul M, Anwar A. Low doses of pristane in comparison with high doses in induction of arthritis in female-sprague dawley rats. Khyber Med Univ J 2016;8(4):185–8.
Faisal R, Shinwari L, Jehangir T. Comparison of the therapeutic effects of thymoquinone and methotrexate on renal injury in pristane induced arthritis in rats. J Coll Physicians Surg Pak 2015;25(8):597–601.
Vingsbo C, Sahlstrand P, Brun JG, Jonsson R, Saxne T, Holmdahl R. Pristane-induced arthritis in rats: A new model for rheumatoid arthritis with a chronic disease course influenced by both major histocompatibility complex and non-major histocompatibility genes. Am J Pathol 1996;149(5):1675–83.
Alam M, Galav V. Anti-inflammatory effect and toxicological evaluation of thymoquinone (volatile oil of black seed) on adjuvant-induced arthritis in Wistar rat. Indian J Life Sci 2013;2(2):17–22.
Umar S, Zargan J, Umar K, Ahmad S, Katiyar C, Khan H. Modulation of the oxidative stress and inflammatory cytokine response by thymoquinone in the collagen induced arthritis in wistarrats. Chem Biol Interact 2012;197(1):40–6.
Tekeoglu I, Dogan A, Ediz L, Budancamanak M, Demirel A. Effects of thymoquinone (volatile oil of black cumin) on rheumatoid arthritis in rat models. Phytother Res 2007;21(9):895–7.
Sajad M, Asif M, Umar S, Zargan J, Rizwan M, Ansari SH, et al. Amelioration of inflammation induced oxidative stress and tissue damage by aqueous methanolic extract of Nigella sativa Linn. in arthritic rats. J Complement Integr Med 2007;7(1):1–21.
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