PROTECTIVE EFFECT OF VITAMIN C ON MONOSODIUM GLUTAMATE INDUCED CHANGES IN THE OVIDUCT OF RATS

Sumaira Abbasi, Saima Mumtaz Khattak, Sana Malik

Abstract


Background: This study was conducted to evaluate the effect of Vitamin C on Monosodium Glutamate induced histopathological changes in oviduct of adult female Sprague Dawley rats. The duration of study was 6 months. It was an analytical experimental randomized control trial. Methods: In this experimental study, 45 female adult Sprague Dawley rats of 10–14 weeks were used and divided into 3 groups. Each group contains 15 rats. Control group (C) received standard laboratory diet. Experimental group A, was given Monosodium Glutamate (0.08 mg/kg body weight/ day) whereas experimental group B, was served on both MSG and Vitamin C (250 mg/kg body weight/day). All groups received diet for a period of 4 weeks. After 4 weeks all rats were sacrificed and oviducts were obtained. For the study of tissue under light microscopy, tissue processing was done by using Haematoxylin and Eosin stain and 5micrometer thick sections were taken from the ampullary part of oviduct. Results: After administration of MSG, group A showed vacuolization of epithelial cells, infiltration of RBCs in lumen with substantial decrease in the diameter of oviduct in group A. Protective effects were seen in vitamin C supplemented group B, with decrease in epithelial vacuolization and RBCs infiltrate along with increase in diameter of oviduct. Conclusion: Vitamin C has protective effect on Monosodium Glutamate induced histological changes in oviduct of rats.

Keywords: diameter of oviduct; MSG; RBC; Vitamin C; Vacuolization

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References


Shaheen R, Subhan F, Sultan S, Subhan K, Tahir F. Prevalence of infertility in a cross section of Pakistani population. Pak J Zool. 2010;42(4):389–93.

Esteves SC, Schattman GL, Agarwal A. Definitions and Relevance of Unexplained Infertility in Reproductive Medicine. In: Unexplained Infertility: Springer, 2015; p.3–5.

Showell MG, Brown J, Clarke J, Hart RJ. Antioxidants for female subfertility. The Cochrane Library. 2013.

Sami N, Ali TS, Wasim S, Saleem S. Risk factors for secondary infertility among women in Karachi, Pakistan. PLoS One 2012;7(4):e35828.

Husarova V, Ostatnikova D. Monosodium Glutamate Toxic Effects and Their Implications for Human Intake: JMED Res 2013;2013:1–12.

Ali AA, El-Seify GH, El Haroun HM, Soliman M. Effect of monosodium glutamate on the ovaries of adult female albino rats and the possible protective role of green tea. Menoufia Med J 2014;27(4):793.

Eweka AO, Eweka A, Om’Iniabohs FA. Histological studies of the effects of monosodium glutamate of the fallopian tubes of adult female Wistar rats. N Am J Med Sci 2010;2(3):146–9.

Waseem N, Butt SA, Hamid S. Amelioration of lead induced changes in ovary of mice, by garlic extract. J Pak Med Assoc 2014;64(7):798–801.

Waseem N, Hamid S, Butt SA. Effect of lead acetate on follicular count of mice and the protective role of garlic extract. Pak Armed Forces Med J 2014;64(1):61–5.

Agarwal A, Allamaneni SS. Role of free radicals in female reproductive diseases and assisted reproduction. Reprod Biomed Online 2004;9(3):338–47.

Zia M, Qamar K, Butt S. Effect of Monosodium Glutamate on the Epithelial Height of Fallopian Tube of Rat and its Prevention with Diltiazem. Pak Armed Forces Med J 2013;1(3):11.

Paulsen G, Cumming KT, Hamarsland H, Børsheim E, Berntsen S, Raastad T. Can supplementation with vitamin C and E alter physiological adaptations to strength training? BMC Sports Sci Med Rehabil 2014;6(1):28.

Zia M, Qamar K, Butt S. Effect of Monosodium Glutamate on the Epithelial Height of Fallopian Tube of Rat and its Prevention with Diltiazem. Pak Armed Forces Med J 2013;1(3):11.

Wahdan RA, Alazouny ZM. Effect of monosodium glutamate on the fallopian tubes of adult albino rats and the possible protective role of vitamin C: a histological and immunohistochemical study. Egypt J Histol 2015;38(1):68–76.

Juraschek SP, Guallar E, Appel LJ, Miller ER 3rd. Effects of vitamin C supplementation on blood pressure: a meta-analysis of randomized controlled trials. Am J Clin Nutr 2012;95(5):1079–88.

Huang HY, Appel LJ, Choi MJ, Gelber AC, Charleston J, Norkus EP, et al. The effects of vitamin C supplementation on serum concentrations of uric acid: results of a randomized controlled trial. Arthritis Rheum 2005;52(6):1843–7.

Barton-Schuster D. Improve your fertility with Vitamin C. [Internet]. [cited 2018 Mar]. Available from: http://natural-fertility-info.com/vitamin-c-to-improve-fertility.html

Mustafa SJ. Effect of Monosodium Glutamate on Mice Ovaries and Possible Protective Role of Vitamin C. Ann Appl Bio-Sci 2015;2(4):A100–5.

Fracassetti D, Costa C, Moulay L, Tomás-Barberán FA. Ellagic acid derivatives, ellagitannins, proanthocyanidins and other phenolics, vitamin C and antioxidant capacity of two powder products from camu-camu fruit (Myrciaria dubia). Food Chem 2013;139(1-4):578–88.

Osman HEH, Elshama SS, E1- Kenaway AE. Study of the role of antioxidant (vitamin c) on modulation toxicity of chronic use of monosodium glutamate in liver of albino rats. Ain Shams J Forensic Med Clin Toxicol 2012;19:75–87.

Soujanya S, Lakshman M, Anand Kumar A, Reddy A. Histopathological and ultrastructural changes induced by imidacloprid in brain and protective role of vitamin C in rats. J Chem Pharma Res 2012;4(9):4307–18.

Bendich A, Machlin LJ, Scandurra O, Burton GW, Wayner DD. The antioxidant role of vitamin C. Adv Free Radic Biol Med 1986;2(2):419–44.


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