COMPARISON OF DIAGNOSTIC EFFICACY OF RAPID DIAGNOSTIC DEVICES FOR DENGUE VIRUS INFECTION-A PIOLT STUDY

Authors

  • Noshin Wasim Yusuf
  • Naeem Kausar
  • Roheena Akbar
  • Nasir Iqbal

Abstract

Background: To compare the diagnostic efficacy of commercially available rapid diagnostic test
devices for Dengue serology. To find out the sensitivity and specificity of rapid diagnostic devices
with Elisa results as the Gold standard. Methods: During the dengue virus epidemic in Lahore a
pilot study was conducted in order to evaluate the diagnostic efficacy of two most frequently used
immunochromatographic rapid test devices in public sector hospitals. The results of both the kits
were compared to each other. Sensitivity and specificity was calculated against results of ELISA
as the reference gold standard. Results: Results of kit-A revealed a very high false negative rate
when compared to ELISA where actual prevalence rate shown by ELISA was 96.0% compared to
prevalence rate of 44.0% with rapid diagnostic test device kit-A. Similarly the results of rapid test
device Kit-B showed high false negative results for dengue virus prevalence. Actual prevalence
rate of dengue fever shown by ELISA was 96.0% where as it turned out to be 50% with the kit-B
rapid test device. Comparison of Results of two kits revealed no significant difference of test
positivity rates. Conclusion: Rapid test devices based on immunochromatographic method
supplied in the public sector hospitals are not reliable diagnostic tools for screening for dengue
virus infection Health authorities need to review their strategy for supply of more reliable tools
during epidemics in order to avoid false negative results.
Keyword: Immunochromatographic rapid test, Dengue virus, ELISA, Screening tests

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Published

2008-12-01

How to Cite

Yusuf, N. W., Kausar, N., Akbar, R., & Iqbal, N. (2008). COMPARISON OF DIAGNOSTIC EFFICACY OF RAPID DIAGNOSTIC DEVICES FOR DENGUE VIRUS INFECTION-A PIOLT STUDY. Journal of Ayub Medical College Abbottabad, 20(4), 26–28. Retrieved from https://jamc.ayubmed.edu.pk/index.php/jamc/article/view/3836

Issue

Vol. 20 No. 4 (2008): JOURNAL OF AYUB MEDICAL COLLEGE, ABBOTTABAD

Section

ORIGINAL ARTICLE

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