RAPID ISOLATION OF MYCOBACTERIA-NEED OF THE HOUR IN OUR SETTINGS

Authors

  • Abdul Hannan
  • Saadia Chaudhary
  • Sidrah Saleem
  • Atika Qayyum
  • Muhammad Usman Arshad

Abstract

Background: To compare Bactec MGIT 960 with LJ media in terms of time taken for the initial
isolation of mycobacteria. Methods: A total of 100 AFB (acid fast bacillus) positive sputum
samples were processed and inoculated in both the Lowenstein Jensen (LJ) media and
mycobacterium growth indicator tube (MGIT) tubes. Results: Of the 100 samples, positive growth
was obtained from all the samples on both the MGIT and LJ media. In MGIT 53% samples grew
in 4 days, 30% in 5 days and 17% in 6 days (Mean=4.6 days) while on LJ media, 44% grew in 30
days, 20% in 35 days and 36% in 44 days (Mean=37 days). Significant difference was observed
between two systems with a p-value of less than 0.05. Conclusion: Bactec MGIT 960 is a much
faster and efficient system for the initial isolation of mycobacteria.
Keywords: Mycobacteria, MGIT, LJ

References

Khosravi AD, Dezfulian A, Alavi SM. Detection of Isoniazid

and Rifampin resistant Mycobacterium tuberculosis isolated

from tuberculosis patients using conventional method and

PCR. Pak J Med Sci 2006;22:47-50.

Rasul S, Shabbir I, Lqbal R, Masood-ul-Haq, Khan SU,

Saeed S, et al. Trends in Multi Drug Resistant Tuberculosis.

Pak J Chest Med 2001;7:21-8.

Dhingra V, Nishi A, Rajpal S. Validity and Reliability of

sputum smear examination as diagnostic and screening test

for Tuberculosis. Ind J Astma Immunol. 2003;17:67-69.

Watterson S, Drobniewski F A. Modern laboratory diagnosis

of mycobacterial Infections. J Clin Pathol. 2000;53:727-32.

Crofton J, Chaulet P, Maher D. Guidelines for the

management of drug resistant tuberculosis. WHO/TB/96.210

Rev1. Geneva: World Health Organization; 1997.

Tenover FC, Crawford JT, Huebner RE, Geiter LJ,

Horsburgh CR Jr, Good RC. The resurgence of tuberculosis:

is your laboratory ready? J Clin Microbiol 1993;31:767-70.

Tortoli E, Cichero P, Piersimoni C, Simonetti MT, Gesu G,

Nista D. Use of BACTEC MGIT 960 for recovery of

mycobacteria from clinical specimens: multicentre study. J

Clin Microbiol. 1999;37:3578-82.

J Ayub Med Coll Abbottabad 2008;20(4)

http://www.ayubmed.edu.pk/JAMC/PAST/20-4/Hannan.pdf 69

Idigoras P, Beristain X, Iturzaeta A, Vicente D, PerezTrallero E. Comparison of the automated nonradiometric

Bactec MGIT 960 system and Lo¨wenstein-Jensen, Coletsos,

and Middlebrook 7H11 solid media for recovery of

mycobacteria. Eur J Clin Microb Infect Dis. 2000;19:350-54.

Telles MAS, Bori A, Amorim ABR, Cruz AF, Pini MIT,

Sato DN. Rapid detection of multidrug-resistant

Mycobacterium tuberculosis using the growth indicator tube

(MGIT) system. Braz J Med Biol. Res. 2002;35:1127-31.

Badak FZ, Kiska DL, Setterquist S, Hartley C, O'Connell

MA, Hopfer RL. Comparison of mycobacteria growth

indicator tube with BACTEC 460 for detection and recovery

of mycobacteria from clinical specimens. J Clin Microbiol

;34:2236-39.

Pfyffer GE, Welscher HM, Kissling P, et al. Comparison of

the Mycobacteria Growth Indicator Tube (MGIT) with

radiometric and solid culture for recovery of acid-fast bacilli.

J Clin Microbiol. 1997;35:364-68.

Published

2008-12-01

How to Cite

Hannan, A., Chaudhary, S., Saleem, S., Qayyum, A., & Arshad, M. U. (2008). RAPID ISOLATION OF MYCOBACTERIA-NEED OF THE HOUR IN OUR SETTINGS. Journal of Ayub Medical College Abbottabad, 20(4), 67–69. Retrieved from https://jamc.ayubmed.edu.pk/index.php/jamc/article/view/3847

Issue

Vol. 20 No. 4 (2008): JOURNAL OF AYUB MEDICAL COLLEGE, ABBOTTABAD

Section

ORIGINAL ARTICLE

License

Journal of Ayub Medical College, Abbottabad is an OPEN ACCESS JOURNAL which means that all content is FREELY available without charge to all users whether registered with the journal or not. The work published by J Ayub Med Coll Abbottabad is licensed and distributed under the creative commons License  CC BY ND Attribution-NoDerivs. Material printed in this journal is OPEN to access, and are FREE for use in academic and research work with proper citation. J Ayub Med Coll Abbottabad accepts only original material for publication with the understanding that except for abstracts, no part of the data has been published or will be submitted for publication elsewhere before appearing in J Ayub Med Coll Abbottabad. The Editorial Board of J Ayub Med Coll Abbottabad makes every effort to ensure the accuracy and authenticity of material printed in J Ayub Med Coll Abbottabad. However, conclusions and statements expressed are views of the authors and do not reflect the opinion/policy of J Ayub Med Coll Abbottabad or the Editorial Board.

USERS are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author. This is in accordance with the BOAI definition of open access.

AUTHORS retain the rights of free downloading/unlimited e-print of full text and sharing/disseminating the article without any restriction, by any means including twitter, scholarly collaboration networks such as ResearchGate, Academia.eu, and social media sites such as Twitter, LinkedIn, Google Scholar and any other professional or academic networking site.