PERIPHERAL BLOOD-BASED POLYMERASE CHAIN REACTION IN DIAGNOSIS OF PULMONARY TUBERCULOSIS
Abstract
Background. The rapid diagnosis of infectious diseases, particularly those that represent a publichealth problem, like tuberculosis, is a challenging problem. By using nucleic acid amplification
techniques like PCR, one may be able to diagnose, the disease on the day of arrival of specimen in
the laboratory. For diagnosis of tuberculosis by direct methods like PCR, specimens from site of
infection are required. In certain cases it is difficult to get the specimens from site of infection and
in such situations; some researchers have tried to detect the DNA of Mycobacterium tuberculosis
complex from blood of these patients. The purposive of this study is to determine the diagnostic
efficacy of peripheral blood-based polymerase chain reaction for diagnosis of pulmonary
tuberculosis. Methods. This was a simple descriptive study, carried out in Department of
Microbiology, Armed Forces Institute of Pathology, Rawalpindi from Jan 2004 to Dec 2004.
Sputum and blood samples were collected from 96 suspected patients of pulmonary tuberculosis.
Sputum samples processed for ZN staining and AFB culture (gold standard) and blood samples
processed for PCR. Results. Out of 96 cases, 60 (62.5%) were culture positive. PCR was positive
in 14 (14.5%). AFB smear positive were 34 (35.4%). The overall sensitivity and specificity of the
PCR assay was 20% and 94.4% respectively and the positive and negative predictive values were
85.71% and 41.46% respectively. The overall efficiency of the test was 47.91%. Conclusion. Due
to low sensitivity; a negative PCR assay does not rule the disease. However, this test may be
helpful in cases where specimens from the site of infection are not available.
Key Words: PCR, peripheral blood, pulmonary tuberculosis
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