• Faiza Amin
  • Shumaila Iqbal
  • Syed Azizuddin


Kingdom of Saudi ArabiaBackground: Contaminated dentures need to be disinfected as a part of denture hygiene regimen. This study was conducted to determine the colour stability of heat cure acrylic resin after treatment with two disinfectants. Methods: This in-vitro experimental study was conducted at Dr Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, Dow University of Health Sciences and Al Karam Textiles Karachi, from January to April 2012. Total 72 rectangular shaped specimens; 18 specimens were measured at baseline (control group) of the study (0 day), 18 specimens were immersed in distilled water. Eighteen (18) specimens were placed in 1% Sodium Hypochlorite solution for 20 minutes and eighteen (18) specimens were placed in Alkaline Gluteraldehyde for 10 minutes. All specimens were polished, stored in distilled water for 24 hours prior to experiment. All the specimens were immersed twice daily for total of 60 days. After 60 days of immersion the specimens were tested for colour changes with spectrophotometer. SPSS-16 was used for statistical analysis. Results: There was statistically significant difference in the colour change (ΔE) between all groups (p<0.001) after 60 days of immersion. At baseline (0 day), trace amount of colour change was observed whereas when specimens immersed in distilled water undergo slight change in colour after 60 days of immersion. Furthermore, specimens immersed in 1% Sodium hypochlorite for 20 minutes and 2%AlkalineGluteraldehyde for 10 minutes for 60 days twice a day showed noticeable changes in colour. Conclusion: There results of the study advocated that the colour stability of denture base acrylic resin is affected by the disinfectants used.Keywords: Heat cure acrylic resin. Colour stability. Spectro photometer. Disinfectants


Sepúlveda-Navarro WF, Arana-Correa BE, Borges CP, Jorge JH, Urban VM, Campanha NH. Colour stability of resins and nylon as denture base material in beverages. J Prosthodont 2011;20(8):632–8.

Powell GL, Runnells RD, Saxon BA, Whisenant BK. The presence and identification of organisms to dental laboratories. J Prosthet Dent 1990;64(2):235–7.

Jnanadev KR, Satish Babu CL, Shilpa Shetty S, Surendra Kumar GP, Sheetal HS. Disinfecting the acrylic resin plate using electrolyzed acid water and 2% glutaraldehyde: a comparative microbiological study. J Indian Prosthodont Soc. 2011;11:36–44.

Chandra J, Patel JD, Li J, Zhou G, Mukherjee PK, McCormick TS, et al. Modification of surface properties of biomaterials influences the ability of Candida albicans to formbiofilms. Appl Environ Microbiol 2005;71(12):8795–801

Brosky ME, Pesun IJ, Morrison B, Hodges JS, Lai JH, Liljemark W. Clinical evaluation of resilient denture liners. Part 2: Candida count and speciation. J Prosthodont 2003;12(3):162–7.

da Silva FC, Kimpara ET, Mancini MN, Balducci I, Jorge AO, Koga-Ito CY. Effectiveness of six different disinfectants on removing five microbial species and effects on the topographic characteristics of acrylic resin. J Prosthodont 2008;17(8):627–33.

Chau VB, Saunders TR, Pimsler M, Elfring DR. In-depth disinfection of acrylic resins. J Prosthet Dent 1995;74(3):309–13.

McCabe JF, Murray ID, Kelly PJ.The efficacy of denture cleansers. Eur J Prosthodont Restor Dent 1995;3:203–7.

McNeme SJ, von Gonten AS and Woolsey GD. Effects of laboratory disinfecting agents on colour stability of denture acrylic resins. J Prosthet Dent 1991; 66:132–6.

Marina Xavier Pisani, Cláudia Helena Lovato da Silva; Helena de Freitas Oliveira Paranhos; Raphael Freitas Souza; Ana Paula Macedo. The Effect of Experimental Denture Cleanser Solution Ricinus communis on Acrylic Resin Properties. Materials Research. 2010;13(3):369–73.

Paranhos Hde F, Davi LR, Peracini A, Soares RB, Lovato CH, Souza RF. Comparison of physical and mechanical properties of microwave-polymerized acrylic resin after disinfection in sodium hypochlorite solutions. Braz Dent J 2009;20(4):331–5.

Devlin H, Kaushik P. The effect of water absorption on acrylic surface properties. J Prosthodont 2005;14:233–8.

Padiyar N, Kaurani P. Colour stability: An important physical property of esthetic restorative materials. Int J Clin Dent Sci 2010; 1:81–4.

Mohamed SH, Al-Jadi AM, Ajaal T. Using of HPLC Analysis for Evaluation of Residual Monomer Content in Denture Base Material and Their Effect on Mechanical Properties. J Phys Sci 2008;19:127–35.

Nimeroff I. Colorimetry. Natl Bureau Stand Monogr 1968;10432.

Pavarina AC, Pizzolitto AC, Machado AL, Vergani CE, Giampaolo ET. An infection control protocol: effectiveness of immersion solution to reduce the microbial growth on the dental prosthesis Oral Rehabil 2003;30(5):532–6.

Silva PM, Acosta EJ, Jacobina M, Pinto Lde R, Porto VC. Effect of repeated immersion solution cycles on the colour stability of denture tooth acrylic resins. J Appl Oral Sci 2011;19(6):623–7.

Koksal T, Dikbas I. Colour stability of different dentureteeth materials against various staining agents. Dent Mater J 2008;27(1):139–44.

Samra AP, Pereira SK, Delgado LC, Borges CP. Colour stability evaluation of aesthetic restorative materials. Braz Oral Res 2008;22(3):205–10.

Craig RG. Restorative Dental Materials, (ed) 12th. St. Louis: Mosby; 2006.chapter no: 3 p 29–30

Reis KR, Bonfante G, Pegoraro LF, Conti PC, Oliveira PC, Kaizer OB. In vitro wear resistance of three types of polymethyl methacrylate denture teeth. J Appl Oral Sci 2008;16(3):176–80.

Ertas E, Güler AU, Yücel AC, Köprülü H, Güler E. Colour stability of resin composites after immersion in different drinks. Dent Mater J 2006;25:371–6.

Yiu CK, King NM, Pashley DH, Suh BI, Carvalho RM, Carrilho MR, et al. Effect of resin hydrophilicity and water storage on resin strength. Biomaterials 2004;25(26):5789–96.

Davenport JC. The oral distribution of Candida in denture stomatitis. Br Dent J 1970;129(4):151–6.

Lee YK, Lim BS, Kim CW. Influence of illuminating and viewing aperture size on the colour of dental resin composites. Dent Mater 2004;20:116–23.

Saraç D, Saraç YS, Kurt M, Yüzbasioglu E. The effectiveness of denture cleansers on soft denture liners coloured by food colourant solutions. J Prosthodont 2007;16:185–91.

Purnaveja S, Fletcher AM, Ritchie GM, Amin WM, Moradians S, Dodd AW et al. Colour stability of two self curing denture base materials. Biomaterials 1982;3:249–50.

Fernandes FH, Orsi IA, Villabona CA. Effects of the peracetic acid and sodium hypochlorite on the colour stability and surface roughness of the denture base acrylic resins polymerised by microwave and water bath methods. Gerodontology. 2013;30:18–25

Ma T, Johnson GH, Gordon GE. Effects of chemical disinfectants on the surface characteristics and colour of denture resins. J Prosthet Dent 1997;77(2):197–204

Hersek N, Canay S, Uzun G, Yildiz F. Colour stability of denture base acrylic resins in three food colourants. J Prosthet Dent 1999;81(4):375–9.