• Munazza Asad
  • Muhammad Aslam
  • Tahir Ahmad Munir
  • Amina Nadeem


Background: To consider new hypoglycaemic, anti-hyperlipidaemic and anti-platelet aggregationsources, aqueous methanol extract of Acacia Nilotica (AN) leaves was investigated in streptozotocininduced diabetic rats. Methods: Diabetes mellitus was induced in 90 out of 120 male albino rats byadministering 50 mg/Kg bodyweight (bw) streptozotocin intraperitonealy, and was confirmed bymeasuring fasting blood glucose level >200 mg/dL on 4th post-induction day. The rats were equallydivided into 4 groups, A (normal control), B (diabetic control), C (diabetics rats treated with plantextract) and group D (diabetics rats treated with glyburide). The rats of group C and D were givensingle dose of 300 mg/Kg bw, AN extract, and 900 µg/Kg bw glyburide respectively for 3 weeks.Blood glucose levels were measured by glucometer, platelet aggregation by DiaMed method, β-thromboglobulin and insulin by ELISA technique, and lipid components were measured by enzymaticcalorimetric method. Results: Significant differences (p<0.05) were noticed in blood glucose, seruminsulin, platelet aggregation and triglyceride levels in diabetic rats treated with AN extract andglyburide as compared to diabetic controlled rats. A significant difference (p<0.05) in β-thromboglobulin and LDL levels was also noticed in rats treated with glyburide than the diabeticcontrolled rats. The levels of fasting blood glucose, β-thromboglobulin and platelet aggregation weresignificantly reduced (p<0.05) in diabetic rats treated with glyburide than AN extract treated rats.Conclusions: Administration of AN leaves extract showed hypoglycaemic and anti-plateletaggregation activity in diabetic rats as that of glyburide.Keywords: Acacia Nilotica, Hypoglycemia, Streptozotocin


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