EFFICACY OF ZINC AS AN ANTIBACTERIAL AGENT AGAINST ENTERIC BACTERIAL PATHOGENS
AbstractBackground: Diarrhoea is a serious threat all over the world with great economic implicationsespecially evident in the developing world. This study was aimed at determining in vitro efficacyof Zinc (Zn) against common enteric bacterial pathogens. Method: A total of 100 bacterial entericpathogens: Salmonellae (n=16), enteropathogenic Escherichia coli (EPEC) (n=26), Shigellae(n=28) and Vibrio cholerae (n=30) were isolated from diarrhoeal stool specimens at Departmentof Microbiology, Armed Forces Institute of Pathology Rawalpindi during Aapril 2009 to Jan 2010.These isolates were tested against various concentrations of Zn supplemented in Mueller Hinton(MH) agar using a multipoint inoculator. A minimum inhibitory concentration of active Zn inZnSO4.7H2O ranging from 0.03 mg/ml to 1 mg/ml was used. Results: Zn completely inhibitedthe growth of all the tested pathogens and most of them were inhibited at a concentration of 0.06mg/ml to 0.5 mg/ml of Zn. Conclusions: Zinc has an excellent antibacterial activity againstenteric bacterial pathogens common in our setup which may provide basis for treatment ofdiarrhoea. Clinical study based on these findings is recommended.Keywords: Diarrhoea, zinc, antibacterial, Enteric Pathogens, Cholera, Salmonella, E. coli, Shigella
Black RE, Morris SS, Bryce J. Where and why are 10 million
children dying every year? Lancet 2003;361:2226–34.
Sur D, Ramamurthy T, Deen J, Bhattacharya SK. Shigellosis:
challenges & management issues. Indian J Med Res
Jafari F, Shokrzadeh L, Hamidian M, Ahrabi SS, Zali MR. Acute
Diarrhoea due to Enteropathogenic Bacteria in Patients at
Hospital in Tehran. Jpn J Infect Dis 2008;61:269–73.
Hoque KM, Binder HI. Zinc in the treatment of acute diarrhea:
Current status and assessment. Gastroenterology 2006;130:2201–5.
Strand TA, Chandyo RK, Bahl R, Sharma PR, Adhikari RK,
Bhandari N, et al. Effectiveness and Efficacy of Zinc for the
Treatment of Acute Diarrhoea in Young Children. Pediatrics
Faruque AS, Mahalanabis D, Haque SS, Fuchs GJ, Habte D.
Double-blind, randomized, controlled trial of zinc or vitamin A
supplementation in young children with acute diarrhea. Acta
Bhutta ZA, Bird SM, Black RE, Brown KH, Gardner JM,
Hadayat A, et al. Zinc Investigators’ Collaborative Group.
Therapeutic effects of oral zinc in acute and persistent diarrhoea
in children in developing countries: pooled analysis of
randomised controlled trials. Am J Clin Nutr 2000;72:1516–22.
Baqui AH, Black RE, Arifeen EI, Yunus M, Chakra-borty J,
Ahmad S, et al. Effect of zinc supplementation started during
diarrhea on morbidity and mortality in Bangladeshi children:
community randomized trial. BMJ 2002;325:1059.
Schreckenberger PC, Linquist D. Algorithms for identification of
Aerobic Gram-Negative bacteria. In: Murray PR, Baron EJ,
Jorgensen JH, Landry ML, Pfaller MA, (eds.). Manual of
Clinical Microbiology, 9th ed, Washington DC: ASM Press,
WHO mortality country fact sheet 2006. Available from:
WHO/UNICEF Joint Statement. Clinical management of acute
diarrhea. Geneva: World Health Organization, 2004. p.7.
Scrimgeour AG, Lukaski HC. Zinc and diarrheal disease: current
status and future perspectives. Curr Opin Clin Nutr Metab Care
Larson CP, Hoque AM, Larson CP, Khan AM, Saha UR.
Initiation of zinc treatment for acute childhood diarrhea and risk
for vomiting or regurgitation: A randomized, double-blind,
placebo-controlled trial. J Health Popul Nutr 2005;23:311–9.
Crane JK, Hoque KM. Zinc for Infectious Diarrhea in Developed
Countries: Should We Be Sprinkling Our Own Lawns? Journal
of Pediatric Gastroenterol Nut 2008;46:484–5.
Sawai J. Quantitative evaluation of antibacterial activities of
metallic oxide powders (ZnO, MgO and CaO) by conductimetric
assay. J Microbiol Methods 2003;54:177–82.
Selahattin A, Kadri G, Ramazan C. Effect of zinc on microbial
growth. Tr J Med Sciences 1998;28:595–7.
Canani R, Ruotolo S. The Dawning of the “Zinc Era” in the
Treatment of Pediatric Acute Gastroenteritis Worldwide? J
Pediatr Gastroenterol Nutr 2006;42:253–5.
Chart H. Escherichia. In: Greenwood D, Slack RC, Peutherer JF,
(eds.). Medical Microbiology A guide to Microbial Infections:
Pathogenesis, Immunity, Laboratory diagnosis and Control, 16th
ed, Philadelphia: Elsevier Inc; 2006. p.265–74.
Ranjbar R, Dallal MM, Pourshaffie MR. Epidemiology of
shigellosis with special reference to hospital distribution of
Shigella strains in Tehran. Iranian J Clin Infect Dis 2008;3:35–8.
Samal SK, Khunita HK, Nanda PK, Satapathay CS, Nayak SR,
Sarangi AK, et al. Incidence of Bacterial Enteropathogens among
Hospitalized Diarrhoea Patients from Orisa, India. Jpn J Infect
Seidlein LV, Kim DR, Ali M, Lee H, Wang XY, Thiem VD, et
al. A Multicentre Study of Shigella Diarrhoea in six Asian
Countries: Disease Burden, Clinical Manifestations and
Microbiology. PLoS Med. 2006;3:e353.
Surjawidaja JE, Hidayat A, Lesmana M. Growth inhibition of
enteric pathogens by Zinc Sulphate: an in vitro study. Med Princ
Iwalokun BA, Bakare S. Comparative sensitivity to Zinc
Sulphate of Shigella isolates recovered from Nigerian children
with low and marginal plasma zinc concentrations. J Pediatr
Infect Dis 2008;3:167–74.
Crane JK, Naeher TM, Shulgina I, Zhu C, Boedeker EC. Effect
of zinc in Enteropathogenic Escherichia coli infection. Infect
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