EFFECT OF DOXORUBICIN AND DAUNORUBICIN ON THE ACTIVITY OF ACETYLCHOLINESTERASE IN ACUTE LYMPHOBLASTIC LEUKAMIA
AbstractBackground: Our study was based on the alteration in the Michaelis Mentin parameters ApparentMichaelis Constant (aKm) and Apparent Maximum Velocity (aVm), which reflects activity ofactylcholinesterase (AChE). This activity decreases in Acute Lymphoblastic Leukaemia (ALL). Thisdecrease in aKm and aVm values shows bad prognosis. Similarly the anticancer drugs likeDaunorubicin and Doxorubicin further decreases the aKm and aVm values which worsen theprognosis. The objective of this study was to determine and compare the extent of inhibition ofAcetylecholine Esterase by Daunorubicin and Doxorubicin in ALL. Methods: Study of 100 patientsincluding both male and female children who’s age ranged from 4 to 8 years and were adviseddoxorubicin and daunorubicin separately were tested by Ellman’s method using acetylcholine iodide assubstrate and 5,5-dithiobis 2-nitrobenzine as a colour reagent regardless of dose regimen i.e. (once in 3week, small dose per week or a continuous infusion for 72 to 96 hours. Results: In this study theMichaelis Mentin parameters Apparent Michaelis Constant (aKm) and Apparent Maximum Velocity(aVm) of the enzyme were estimated both in normal individuals and in the patients and also duringtreatment with daunorubicin and doxorubicin. The value of Michaelis Mentin parameters, aKm, aVmand percentage activity of the enzyme in normal individual are 23, 70, and 100 respectively. The valuesof aKm, aVm and percentage activity of the enzyme were also estimated in the patients before and aftertreatment. The values of aKm and aVm in patients of acute lymphoblastic leukaemia and percentageactivity of enzyme is decreased. After the treatment with daunorubicin and doxorubicin the values andactivity is further decreased. Conclusion: We conclude that the drugs under study both decrease theenzyme activity but daunorubicin inhibits the enzyme more than doxorubicin.Keywords: Doxorubicin, daunorubicin, acetylcholinestrase
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