HELICOBACTER PYLORI DETECTION IN CHRONIC GASTRITIS: A COMPARISON OF STAINING METHODS
AbstractBackground: Helicobacter pylori is an important cause of chronic gastritis, gastric ulceration andgastric malignancies as gastric carcinoma and MALT lymphoma. Its definitive diagnosis is basedon histopathology. Routine H & E stain is not very effective in its detection, immune-stains andfluorescent stains are costly. Need for simple cheap and sensitive stain has always been a topic ofhot debate and extensive research. Method: paraffin embedded blocks of all adult patientsdiagnosed as chronic gastritis/gastric ulceration with no accompanying gastric pathology ashypertrophic gastropathys, and neoplasias were taken into study. Three sections of 4 micron werecut and stained with routine H & E, Giemsa, and Cresyl fast violet. Results: Total number ofpatients was 50. Out of these 37 (74%) were males and 13 (26%) were females. Mean age of thepatients was 50.4 years. Thirty-four percent (34%) were positive in normal H & E stain, 68% werepositive in Giemsa and 76% were positive in Cresyl fast violet. Conclusion: Cresyl fast violet is agood stain for diagnosis of H. pylori gastritis.Keywords: H pylori, chronic gastritis, H pylori staining methods
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