• Nadia Mahmood
  • Tahira Naseem
  • Farhana Mukhtar
  • Rubina Basheer


Background: Coronary diseases appear to result from an overbalance between radical-generating,compared with radical-scavenging systems, a condition called as oxidative stress. Total antioxidant status(TAS) in human plasma reflects the balance between oxidants and antioxidants in each system. Bilirubinhas been considered an antioxidant, with capacity to remove reactive species of oxygen. Present studytried to measure the total antioxidant status of first degree relatives of patients with IHD. Study also triedto evaluate the prognostic role of serum bilirubin in disease prevention or progression. Methods: Seventyfive apparently healthy subjects in age group 20–50 years, comprising equal number of males andfemales, who were first degree relatives of ischemic heart disease patients, were included in the study.Family members were divided on the bases of their numbers, i.e., one family member (Group-A), 2 familymembers (Group-B) and more than 3 family members (Group-C). Study was cross sectional and carriedout in a period of 6 months (Jun 2008–Jan 2009). Subjects with letter of consent were taken from generalpopulation. Seventy five healthy age matched people with no history of ischemic heart disease in familywere taken as control. An overnight fasting blood sample was taken. Total antioxidant status wasdetermined using a commercially available kit. Serum bilirubin was estimated by auto analyzer. Results:Family history of ischemic heart disease with serum bilirubin showed a significant negative correlation(p<0.05). But the values of TAS failed to show any significant correlation with the family history. It wasobserved that the value of serum bilirubin was decreased significantly (p<0.05) with an increased numberof family members. Total antioxidant status failed to show any significant difference among all the threegroups. Conclusion: Our data demonstrated that reduced serum levels of bilirubin were seen in peoplewith a higher prevalence of coronary artery disease in the family. The levels of serum bilirubin showed adownward trend with an increase in number of family members affected with ischemic heart disease.Present study failed to show a definite association of total antioxidant status with family history ofischemic heart disease. Additional studies are still necessary on large number of first degree relatives toconfirm and demonstrate the association of these findings with clinical outcomes.Keywords: Ischemic heart disease, first degree relatives, total antioxidant status and bilirubin


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