• Muhammad Jan


Background: Peptic ulcer is mostly produced due to the over production of gastric acid. This study wasundertaken to find out the effects of extract from the leaves of medicinal plant Olea europea (whichcontains documented natural Calcium channel blocker) on volume and acidity of Carbachol inducedgastric section. Its effects were also observed on liver and kidney function. Methods: Thirty rabbits oflocal breed, weighing 1–1.5 kg were used. The animals were kept on fasting for 48 hours, after that thepylorus of each animal was ligated. Carbachol 600 μg/kg body weight and extract from the leaves ofOlea europea 500 mg/kg body weight were administered intraperitoneally to group A and Brespectively. The extract was also administered in the same dose to group C for 45 days twice dailyintraperitoneally to observe its effects on liver and kidney function. Results: The extract reduced thevolume, free and total acidity of gastric secretion, which were statistically highly significant whencompared with Carbachol (p<0.001). When the differences of means for both the liver and kidneyfunction were compared with that of control group, all these were found statistically non-significantindicating that the extract has no adverse effects on these organs regarding all parameters included instudy. Conclusion: Extract can be used effectively and safely in the treatment of hyper gastric acidityconditions and peptic ulcer after evaluation of its effects in human subjects.Keywords: Medical plants, Olea Europea


Edward CRW, Bouchier IAD, Haslett C. Diseases of the

stomach. In: Davidson’s Principles and Practice of Medicine,

London: Churchill Livingstone; 1995.p.425–34.

Azhar I, Aftab K, Usmanghani K. Naturally occurring calcium

channel blockers. Hamdard Med 1995;38(2):5–16.

Rauwald HW, Brehm O. Screening of some medicinal plants for

their possible calcium antagonistic activity. Planta Med


Basso N, Materia A, Folini A, Jafe BM. Prostaglandin generation

in the gastric mucosa of rats with stress ulcer. Surgery


Passaro E J, Basso N, Walsh JH. Calcium challenge in

Zollinger-Ellision syndrome, Surgery 1972;72:60–7.

Vischer FE, Seay PH, Tazelaar AP, Veldkamp J W, Brook MJ.

Pharmacology of pamine Bromide. J Pharmacol Expt Ther


Varley H. Test of gastric function, occult blood. In practical

clinical biochemistry, London: Willium Meinmann; 1962.p.


Shambuerk RD, Schubert ML. Control of gastric acid secretion.

Gastroenterol Clin North Am 1992;21(3):527–50.

Negulescu PA, Matchen TE. Intracellular calcium regulation

during secretogogues stimulation of the parietal cells. Am J

Physiol 1988;254:130–40.

Brogden RN, Carmine AA, Heel RC, Speight TM, Avery GS.

Ranitidine: A review of its pharmacology and therapeutic use

in peptic ulcer disease and other allied diseases. Drug


Brage R, Cortijo J, Esplugues JK, Esplugues J, Bomnati EM,

Rondriguez C. Effects of calcium channel blockers on gastric

emptying and acid secretion of the rat in vivo. Br J Pharmacol


Kirkegaard P, Charistianson J, Peterson B, Olsen PS. Calcium

and stimulus-secretion coupling in gastric fundic mucosa: effect

of inhibition of calcium transport by verapamil on gastric acid

secretion in the isolated guinea pig fundic mucosa and in healthy

subjects. Scand J Gastroenterol 1982;17:533–8.

Rogers C, Pihan G, Szabo S. Role of leukotrienes in the

pathogenesis of haemorrhagic gastric mucosal lesions induced by

ethanol or HCL in the rat. Gastroenterol 1986; 90:1797.

Main IHM, Pearce JB. Effects of calcium on acid secretion from

the rat isolated gastric Mucosa during stimulation with histamine,

pentagastrin, methacholine, and dibutyrl cyclic adenosine-3,5-

monophosphate. Br J Pharmacol 1978;64:359–68.



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