• Saima Bukhari Department of Pharmacology, Ayub Medical College, Abbottabad
  • Sadia Chiragh Department of Pharmacology, Post-graduate Medical Institute, Lahore
  • Sumbal Tariq Department of Pharmacology, Ayub Medical College, Abbottabad
  • Muhammad Adeel Alam Department of Pharmacology, Ayub Medical College, Abbottabad
  • Muhammad Salim Wazir Department of Community Medicine, Ayub Medical College, Abbottabad
  • Muhammad Suleman Department of Epidemiology and Biostatistics, Hazara University, Mansehra


Background: Urinary tract infection is the most common bacterial infection in the community, mainly caused by Escherichia coli (E coli). Due to its high incidence and recurrence, problems are faced in the treatment with antibiotics. Cranberry being herbal remedy have long been the focus of interest for their beneficial effects in preventing urinary tract infections. This study was conducted to analyse in vitro activity of cranberry (Vaccinium macrocarpon) on uropathogenic E coli in uncomplicated urinary tract infections. Methods: In this laboratory based single group experimental study, anti-bacterial activity of Vaccinium macrocarpon concentrate on urinary tract E coli was investigated, in vitro. Ninety-six culture positive cases of different uropathogens were identified. Vaccinium macrocarpon concentrate at different concentrations was prepared in distilled water and put in wells punched in nutrient agar. E coli isolates were inoculated on the plates and incubated at 37 oC for 24 hours. A citric acid solution of the same pH as that of Vaccinium macrocarpon was used and put in a well on the same plate to exclude the effect of pH. Results: A total of 35 isolates of E coli were identified out of 96 culture positive specimens of urine and found sensitive to Vaccinium macrocarpon (p< 0.000). Results revealed that Vaccinium macrocarpon has antibacterial effect against E coli. Furthermore the antibacterial activity of Vaccinium macrocarpon has dose response relationship. Acidic nature of Vaccinium macrocarpon due to its pH is not contributory towards its antibacterial effect. Conclusion: Vaccinium macrocarpon concentrate may be used in urinary tract infection caused by E coli.


Nicolle LE. Uncomplicated urinary tract infection in adults including uncomplicated pyelonephritis. Urol Clin North Am 2008;35(1):1–12.

Acharya A, Gautam R, Subedee L. Uropathogens and their antimicrobial susceptibility pattern in Bharatpur, Nepal. Nepal Med Coll J 2011;13(1):30–3.

Akortha EE, Ibadin OK. Incidence and antibiotic susceptibility pattern of staphylococcus aureus amongst patients with urinary tract infection (UTI) in UBTH Benin city, Nigeria. Afr J Biotech 2008;7(11):1637–40.

Palmer RM. Urinary tract infection. 2011. [Internet] [Cited 2015 Jan 17] Available from:

Bilgic D, Beji NK. Lower urinary tract symptoms in women and quality of life. Int J Urol Nurs 2010;4(3):97–105.

Schmiemann G, Kniehl E, Gebhardt K, Matejczyk MM, Hummers-Pradier E. The diagnosis of urinary tract infection: a systematic review. Deut Ärzteblatt Int 2010;107(21):361–7.

Lane DR, Takhar SS. Diagnosis and management of urinary tract infection and pyelonephritis. Emergen Med Clin North Am 2011;29(3):539–52.

Baker LRJ. Renal disease. In: Kumar, P. J. and Clark, M. eds. 2004. Clinical medicine. London: Elsevier. 2004. Ch. 11 p.616.

Schwartz BS. Urinary tract infections. In: Levinson, W. 2014. Rev Med Microbiol Immunol. USA: LANGE. Ch. 78. p.561–72.

Foxman B, Barlow R, D'Arcy H, Gillespie B, Sobel JD. Urinary tract infection: self-reported incidence and associated costs. Ann Epidemiol 2000;10(8):509–15.

Farhat-Ullah, Malik SA, Ahmed J. Antibiotic susceptibility pattern and ESBL prevalence in nosocomial Escherichia coli from urinary tract infections in Pakistan. Afr J Biotechnol 2009;8(16):3921–6.

Minardi D, d’Anzeo G, Cantoro D, Conti A, Muzzonigro G. Urinary tract infections in women: etiology and treatment options. Int J Gen Med 2011;4:333–43.

Hisano M, Bruschini H, Nicodemo AC, Srougi M. Cranberries and lower urinary tract infection prevention. Clinics (Sao Paulo) 2012;67(6):661–8.

Micali S, Isgro G, Bianchi G, Miceli N, Calapai G, Navarra M. Cranberry and recurrent cystitis: more than marketing? Crit Rev food Sci Nutr 2014;54(8):1063–75.

Lavigne JP, Bourg G, Combescure C, Botto H, Sotto A. In‐vitro and in‐vivo evidence of dose‐dependent decrease of uropathogenic Escherichia coli virulence after consumption of commercial Vaccinium macrocarpon (cranberry) capsules. Clin Microbiol Infect 2008;14(4):350–5.

Pérez‐López FR, Haya J, Chedraui P. Vaccinium macrocarpon: an interesting option for women with recurrent urinary tract infections and other health benefits. J Obstet Gynaecol Res 2009;35(4):630–9.

Tanvir R, Hafeez R, Hasnain S. Prevalence of multiple drug resistant Escherichia coli in patients of urinary tract infection registering at a diagnostic laboratory in Lahore Pakistan. Pak J Zool 2012;44(3):707–12.

Ng SY, Kwang LL, Tan TY. Identification of Gram-negative bacilli directly from positive blood culture vials. J Med Microbiol 2007;56(Pt 4):475–9.

Greenwood D, Ogilvie M. Antimicrobial agents. In: Greenwood, D., Slack, R., Peutherer, J. and Barer, M. (Eds). 2007. Medical Microbiology. 17th ed. Edinburgh: Churchill livingstone, p.65.

Wu VC-H, Qiu X, Bushway A, Harper L. Antibacterial effects of American cranberry (Vaccinium macrocarpon) concentrate on foodborne pathogens. LWT-Food Sci Technol 2008;41(10):1834–41.

Theodros G. Bacterial pathogens implicated in causing urinary tract infection (UTI) and their antimicrobial susceptibility pattern in Ethiopia. Revista CENIC. Ciencias Biológicas 2010;41:1–6.

Demilie T, Beyene G, Melaku S, Tsegaye W. Urinary bacterial profile and antibiotic susceptibility pattern among pregnant women in North West Ethiopia. Ethiop J Health Sci 2012;22(2):121–8.

Desai P, Ukey PM, Chauhan AR, Malik S, Mathur M. Etiology and antimicrobial resistance pattern of uropathogens in a hospital from suburban mumbai. Int J Biol Med Res 2012;3(3):2007–12.

Ahmad W, Jamshed F, Ahmad W. Frequency of escherichia coli in patients with community acquired urinary tract infection and their resistance pattern against some commonly used anti bacterials. J Ayub Med Coll Abbottabad. 2015;27(2):333–7.

Sengupta K, Alluri K, Golakoti T, Gottumukkala G, Raavi J, Kotchrlakota L, et al. A randomized, double blind, controlled, dose dependent clinical trial to evaluate the efficacy of a proanthocyanidin standardized whole cranberry (Vaccinium macrocarpon) powder on infections of the urinary tract. Curr Bioact Compounds 2012;7(1):39–46.

Howell AB, Botto H, Combescure C, Blanc-Potard AB, Gausa L, Matsumoto T, et al. Dosage effect on uropathogenic Escherichia coli anti-adhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content: a multicentric randomized double blind study. BMC Infect Dis 2010;10:94.

Tempera G, Corsello S, Genovese C, Caruso FE, Nicolosi D. Inhibitory activity of cranberry extract on the bacterial adhesiveness in the urine of women: an ex-vivo study. Int J Immunopathol Pharmacol 2009;23(2):611–8.

Gupta K, Chou MY, Howell A, Wobbe C, Grady R, Stapleton, AE. Cranberry products inhibit adherence of p-fimbriated Escherichia coli to primary cultured bladder and vaginal epithelial cells. J Urol 2007;177(6):2357–60



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