IN VITRO PHAGOCYTOSIS OF METHICILLIN RESISTANT AND METHICILLIN SENSITIVE STAPHYLOCOCCUS AUREUS BY HUMAN POLYMORPHONUCLEAR LEUCOCYTES

Authors

  • Numan Javed
  • Nadeem Afzal
  • Romeeza Tahir
  • Afia Abbas

Abstract

Background: Staphylococcus aureus is a gram positive bacterium that causes a number of diseasessuch as abscesses, infective endocarditis, septic arthritis, etc. It is acquiring resistance against manyantibiotics like methicillin; therefore its control is becoming increasingly difficult. Peripheral bloodphagocytes particularly polymorphonuclear leucocytes play an important role in the protectivemechanisms against these organisms. Phagocytes interact with bacteria and phagocytose thesemicroorganisms to kill them. Methods: Phenotypically different isolates of Staphylococcus aureusincluding methicillin resistant Staphylococcus aureus (MRSA) and methicillin sensitiveStaphylococcus aureus (MSSA) were collected from various hospitals of Lahore, Pakistan. Freshpolymorphonuclaer leucocytes were obtained from healthy individuals by centrifugation using FicolHypaque gradient combined with dextran sedimentation. Microbiological method was used for thedetermination of phagocytic index of phenotypic variants of Staphylococcus aureus. Results: Asignificant difference was observed between the phagocytic index of both bacterial groups. MSSAgroup showed the Mean±SD of 79.46%±3.9 while MRSA group showed 72.35%±2.5. Conclusion:Significant difference in phagocytic index indicates that it can be one of the mechanisms of MRSAto evade host immune system as compare to MSSA.Keywords: MRSA, MSSA, Phagocytic index, Polymorphonuclear leucocytes

References

Joachim D. Methicillin resistant Staphylococcus aureus

(MRSA): Diagnostic, clinical relevance and therapy. J Dtsch

Dermatol Ges. 2009. Available online at:

http://www3.interscience.wiley.com/journal/122242047

Garau J, Bouza E, Chastre J, Gudiol F, Harbarth S.

Management of methicillin- resistant Staphylococcus aureus

infections. Clin Microbiol Infect 2009;15:125–36.

Deurenberg RH, Stobberingh EE. The evolution of

Staphylococcus aureus. Infect Genet Evol 2008;8:747–63.

Ito T, Ma XX, Takeuchi F, Okuma K, Yuzawa H, Hiramatsu

K. Novel type V staphylococcal cassette chromosome mec

driven by a novel cassette chromosome recombinase, ccrC.

Antimicrob Agents Chemother 2004;48:2637–51.

Lieberman JM. Appropriate antibiotic use and why it is

important: the challenges of bacterial resistance. Pediatr

Infect Dis J 2003;22:1143–51.

Janeway J, Medzhitov R. Innate immune recognition. Annu

Rev Immunol 2002;20:197–16.

Choi EY, Santoso S, Chavakis T. Mechanisms of neutrophil

transendothelial migration. Front Biosci 2009;14:1596–605.

DeLeo FR, Diep BA, Otto M. Host defense and pathogenesis

in Staphylococcus aureus infections. Infect Dis Clin North

Am 2009;23:17–34.

Cunnion KM, Zhang HM, Frank MM. Availability of

complement bound to Staphylococcus aureus to interact

with membrane complement receptors influences efficiency

of phagocytosis. Infect Immun 2003;71:656–62.

Rooijakkers SH, Van KP, Van SJ. Staphylococcal innate

immune evasion. Trends Microbiol 2005;13:596–601.

J Ayub Med Coll Abbottabad 2009;21(2)

http://www.ayubmed.edu.pk/JAMC/PAST/21-2/Numam.pdf

Kantari C, Pederzoli M, Witko SV. The role of neutrophils

and monocytes in innate immunity. Contrib Microbiol

;15:118–46.

Annemick BS, Jeanette HW. Mac-1 (CD11b/CD18) is

essential for Fc receptor-mediated neutrophil cytotoxicity and

immunologic synapse formation. Blood 2001;97:2478–86.

Ofek I, Keisari J, Sharon N. Nonopsonic phagocytosis of

microorganisms. Annu Rev Microbiol 1995;49:239–76.

Witko-Sarsat V, Rieu P, Descamps-Latscha B, Lesavre P,

Halbwachs-Invest L. Neutrophils: molecules, functions and

pathophysiological aspects. Lab 2000; 80:617–53.

Chambers HF. The changing epidemiology of

Staphylococcus aureus. Emerg Infect Dis 2001;7:178–82.

Salgado MM, Pignatari AC, Bellinati-Pires R. Phagocytosis

and Killing of Epidemic Methicillin-Resistant

Staphylococcus aureus by Human Neutrophils and

Monocytes. Braz J Infect Dis 2004;8:80–9.

Pramanik T, Thapa M, Saikia TC. Effect of temperature on

phagocytic activity of neutrophils. Nepal Med Coll J

;6:39–40.

Bukovsky M, Koscova H, Dubnickova M, Sirotkova L.

Comparative study of disintegrated cells influence of

Staphylococcus aureus, Escherichia coli and Candida

albicans on human and mouse immune mechanisms. Bratisl

Lek Listy 2001;102:314–7.

Peacock JE, Moorman DR, Wenzel RP, Mandell GL.

Methicillin-resistant Staphylococcus aureus: Microbiologic

characteristics, antimicrobial susceptibilities, and assessment

of virulence of an epidemic strain. J Infect Dis

;144:575–82.

Jordens JZ, Duckworth GJ, Williams RJ. Production of

“virulence factors” by “epidemic” methicillin-resistant

Staphylococcus aureus in vitro. J Med Microbiol

;30:245–52.

Mekontso-Dessap A, Honoré S, Kirsch M, Plonquet A,

Fernandez E, Touqui L, et al. Blood neutrophil bactericidal

activity against methicillin-resistant and methicillin-sensitive

Staphylococcus aureus during cardiac surgery. Shock

;24:109–13.

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Published

2009-06-01