PREVENTIVE ROLE OF ZINC CHLORIDE AGAINST TOXICITY OF CIPROFLOXACIN ON THE GROWING CARTILAGE OF WISTAR ALBINO RAT LITTER
AbstractObjective: To assess the preventive role of zinc chloride on toxicity of ciprofloxacin administration inwistar albino rat litter. It was a Prospective experimental study. The study was carried out in thedepartment of Anatomy, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre,Karachi, Pakistan during March 2002 to February 2003 one year study. Method: Ciprofloxacin andzinc chloride were administered to newly born albino rat litters separately and simultaneously at a doseof 20 mg/kg body weight and 1200 g/Kg body weight respectively, intraperitonealy twice daily from1–14 day after birth. The animals were sacrificed by deep ether anaesthesia. The fore and hind limbswere dis-articulated from the axial skeleton, soft tissue was removed and bones were fixed in 10%buffered formalin. Decalcification was done in 10% nitric acid and 10% formic acid changes. Afterparaplast embedding, 4m thick longitudinal sections of proximal & distal ends of long bones were cutby a rotary microtome. Routine staining with haemotoxylin and eosin was performed.Histomorphometery was done to measure the thickness of epiphyseal cartilage and was compared withsimilar values of the control animals. The results were statistically analyzed to evaluate the significance.Result: Our study revealed that ciprofloxacin administration in new born albino rat litter decreased thewidth of epiphyseal growth plate cartilage by 13.7±0.42 m, 10.43% in humerus and 6.6±1.2 m4.72% in femur as compared to control, whereas, simultaneous zinc chloride administration restrictedthe decrease to 1.27 m±SD in humerus and 2.05 m±SD in femur. Conclusion: Simultanous zincchloride administration minimized the epiphseal cartilage damage induced by ciprofloxacin in Wistaralbino rat litter.Keywords: Ciprofloxacin, Cartilage toxicity, Cartilage growth, Zinc chloride, Epiphyseal growthplate, Growth rate, Chondrocyte.
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