• Imranud Din Khattak
  • Taj Muhammad Khan
  • Purdil Khan
  • Syed Mukhtar Ali Shah
  • Sania Tanveer Khattak
  • Anwar Ali


Background: Up till now about 400 red cells antigen have been identified. The majority are inheritedby Mendelian Fashion. The ABO blood group system was first to be identified and RH blood groupsystem was the 4th one, both are most important for blood transfusion purposes. This study is conductedto determine the frequency of ABO and Rhesus (Rh) blood groups in District Swat, NWFP, Pakistan. Itis a cross sectional prospective study and was conducted at Saidu Teaching Hospital district Swat, overa period of one year. (1st Jan, 2007 to 31st Dec, 2007). Methods: A total of 22897 subjects wereincluded in this study. Patients were collected from different wards of Saidu Teaching Hospital whilethe donors from common population. From each subject blood was collected, ABO and Rh bloodgrouping were carried out by tile method using commercially prepared anti sera. The frequency of eachtype was calculated. Results: Out of 22897 subjects 17141 (74.86%) were male subjects and 5756(25.14%) were female. Out of 17141 male subjects 15597 (90.99%) and out of 5756 female subjects5040 (87.56%) were found to be Rh-positive. The frequency of Rh-negative group in male subjectswere (9.01%) where as in female subjects were (12.22%). The frequency of A, B, O and AB groups inRh-positive male subjects were 25.63%, 29.54%, 26.04% and 9.78%, amongst female subjects, it was24.53%, 28.06%, 25.54% and 9.43% respectively. In Rh-negative male subjects the frequency of A, B,O and AB is 2.25%, 2.88%, 3.01% and 0.88%, while amongst females it is 3.54%, 4.24%, 3.74% and0.92% respectively. Conclusion: It is concluded from this study that frequency of Rh-positive bloodgroup is B, O, A, and AB in both gender. Where as the most common Rh-negative in male and femalesubjects are O, B, A, AB, and B, O, A, and AB respectively.Keywords: ABO blood group, Rh blood group, Swat, Pakistan


Hoffbrand AV and Pettit JE, Blood Transfusion .In Essential

Haematology, 5th Edition, Oxford UK: Black well scientific

Publication , 2006;307– 9.

Garraty G, Dzik W,Issitt PD, Lubin DM, Reid ME, Zelinski T.

Terminology for blood group antigens and genes-historical

origins and guideline in the new millennium. Transfusion


Rahman M and Lodhi Y. Frequency of ABO and Rhesus blood

groups in blood donors in Punjab. Pak J Med Sci 2004;20:315–8

Alam M. ABO and Rhesus blood groups in potential blood

donors at Skardu (Northern Areas). Pak J Pathol 2005;16:94–7

Khan MS, Subhan F, Tahir F, Kazi BM, Dil AS, Sultan S.

Prevalence of blood groups and Rh factor in Bannu region

NWFP (Pakistan). Pak J Med Res 2004;43(1):8–10.

Khaliq MA, Khan JA, Shah H, Khan SP. Frequency of ABO and

Rh (D) blood group in Hazara division (Abbottabad). Pak J Med

Res 1984;23:102–3.

Khurshid B, Naz M, Hassan M, Mabood SF. Frequency of ABO

and Rh (D) blood groups in district Sawabi NWFP Pakistan. J

Sci Tech Univ. Peshawar 1992;16:5–6.

Mollison PL,Engelfriet CP,Conteras M : Immunology of red

cells. In Blood Transfusion in Clinical Medicine, 9th Edition

Oxford: Blackwell Scientific Publication, 1993;p.87–8.

Goodnough LT, Brecher ME ,Kater MH, AuBuchon JP.

Transfusion medicen.l. Blood transfusion. N Engl J Med


Majeed T, Hayee A. Prevalence of ABO blood group and sub

groups in Lahore, Punjab (Pakistan). Biomedica 2002;18:11–5.

Zafar NJ, Hasan K, Bukhari K. Prevalence of ABO and Rh blood

group amongst voluntary blood donors. J Rawal Med Coll


Mian A and Farooq A. Distribution of ABO and Rh blood group

alleles in different population of Southern Punjab. Pakistan

anthropl Anz, Man 1999;57:33–9.

Hussain A, Shiekh SA, Haider M, Rasheed T, Malik MR.

Frequency of ABO and Rh blood groups in population of

Balouchistan (Pakistan). Pak Armed Forces Med J


Khaskheli DK ,Qureshi AH, Akhund AA. Distribution of ABO

and Rh groups in the residents of Sindh. Pak J Health


Amjad H, Wajahat H, Janbaz A, Fazli Rabbi and Javed AQ .

Prevalence of phenotypes and Genes of ABO and Rhesus (Rh)

blood groups in Faisalabad , Pakistan. Pak J Biological Sci


Bhatti R,Shiekh DM . Variations of ABO Blood groups. Gene

Frequencies in Population of Sindh, (Pakistan).Ann King Edward

.Med Coll 1999;5:328–31.

Das PK, Nair SC, Harris VK , Rose D, Mammen J, Bose YN and

et al. A Distribution of ABO and Rh–D blood groups among

blood donor in a tertiary care centre in South India. Trop Doct


Tomilan VV, Gurtovaia SW. The incidence of finding ABO

system antigens in the population of the Russian Federation. Sud

Med Ekspert 1999;42:16–8.

Mollison PL, Engelfriet CP. ABO, Lewis, Ii and P Groups. In:

Blood Transfusion in Clinical Medicine, 9th Edition. Oxford:

Blackwell Scientific Publication, 1993;p.150–1.

Frances TF: Blood groups (ABO groups). In: Common

Laboratory and Diagnostic Tests. 3rd Edition, Philadelphia:

Lippincott, 2002;p.19–5.

Bashwari LA, Al Mulhim AA, Ahmad MS, Ahmed MA.

Frequency of ABO blood groups in Eastern region of Saudi

Arabia . Saudi Med J 2001;22:1008–12.

Marzban M, Kamali MS, Hosseinbasi T. Blood groups of the

people of Ahwaz , Iran. Anthropol Anz 1988;46(1):83–9.

Mollison PL, Engelfriet CP, Conteras M. The Rh blood Group

system.In Blood Transfusion in Clinical Medicine, 9th Edition.

Oxford: Black well Scientific Publication, 1993;2008–9.



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