ANTIBIOGRAM STUDIES OF SALMONELLA ENTERITIDIS PHAGE TYPE 4 ISOLATES FROM POULTRY AND MEAT
AbstractBackground: Human infection with Salmonella enteritidis phage type 4 has increased worldwide since last decade and has been shown to be related mainly with the consumption of poultry meat and eggs. The public health significance and economic importance of this serovar underscores the need to generate base line data on the antimicrobial susceptibilities and protein profile of indigenous S. enteritidis. This study was performed to investigate the antimicrobial susceptibilities of S. enteritidis PhageType4 isolates from poultry and meat. Method: This study was carried out in the department of biological sciences Quaid-i-Azam University Islamabad during 1998-2000. A total of nineteen quinolone sensitive isolates of S. enteritidis from poultry meat and eggs collected during1994-1998 were characterized. The isolates were serotyped and phage typed at federal institute of consumer Health and Veterinary Medicine, Wernigerode Germany. Antimicrobial susceptibility tests were performed in accordance to the method of Bauer et al. (1966). Results: The results of the standard disc diffusion test showed 100% resistance against bacitracin, erythromycin and novobiocin. All (100%) isolates were highly sensitive to chloramphenicol. The results of minimum inhibitory concentration (MICs) tests using serial dilution of antimicrobial drugs revealed that 100% of the isolates were resistant against bacitracin, erythromycin and novobiocin at various levels of concentrations. Kanamycin, streptomycin and spectinomycin, all had very poor activity against serovar Enteritidis. Conclusion: These findings suggest the limited therapeutic potential and low typability of this serovar.Key Words: Antibiogram, Salmonella Enteritidis, Phage Type 4.
Gracia del portillo F, Finlay BB. Invasion and intracellular proliferation of Salmonella within non-pathogenic cells. Microbiologia. SEM 1994;10: 229-38.
Rampling AA. Salmonella enteritidis five years on. Lancet 1993;342:317-8.
Mishu B, Kohler J, Lee LA. Out break of Salmonella enteritidis infections in the United States, 1985-1991. Journal of Infections Disease 1994;169: 547-52.
Rodrigue DC. International increase in Salmonella enteritidis. A new pandemic?Epidemiol Infect 1990;105:21-7.
Rubino S, Muresu E, Santona M, Paglietti B, Azara A, Schiaffino A et al. IS 200-finger printing of Salmonella enterica. Epidmiol Infect 1998;120:215-22.
Terajima J, Nakamura A, Watanabe H. Epidemiological analysis of Salmonella enterica, serovar Enteritidis isolates in Japan by phage typing and pulsed field gel-electrophoresis. Epidemiol Infect 1998;120:223-9.
Sumalee-Bangtrakulnonth BA, Pornrunangwong S, Terajima J, Watanable H, Ichikaneko K, Ogawa M. Epidemiological analysis of Salmonella enteritidis 1998; 36(4): 971-974.
ChrongKhan MC, Van T, Lin W, Lin J. PCR Assay For Rapid Detection of Salomonella spp in Foods. PRA-SW, ORA, FDA, Los Angeles CA 900915. 2002.
Bauer KA, Sherris J, Turk M. Antibiotic Susceptibility testing by standardized single disc method. Am J Clin Pathol 1966; 45: 493-6.
Singer, JT, Opitz HM, Gershman M, Hall MM, Moniz IG, Rao SV. Molecular characterization of Salmonella enteritidis isolates from main poultry and poultry farm environment. Avian Dis 1992;36:324-33.
Parkash, Pilli. Multiple resistant S.typhi in India. APUA Newslett 1992; 10.
Siddique BM, Bucar E. Antibiotic sensitivity of Salmonella isolated from poultry. Arch.Roum.path.Exp. Microbiol 1985;44; 301-9.
Lamb VA, Mayhall CG, Spadora AC, Dalton HP. Out break of Salmonella typhimurium gastroenteritis due to an imported strain resistant to ampicillin, chloramphenicol, trimethoprim-sulphamethoxazole in a nursery. J Clin Microbiol 1984; 20;1076-9.
Sultana K, Bushra MA, Nafisa I. Evaluation of antibiotic resistance in clinical isolates of Salmonella typhi from Islamabad Pakistan. Zool 1995; 27(2): 185-7.
Mansoor A. Molecular characterization of Avian Salmonella isolates. M.Phil. Thesis. Department of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan. 1997.
Ikram N. Molecular epidemiology of Salmonella isolates obtained from local hospital in Islamabad. M.Phil. Thesis, Department of Biological Sciences, Quaid-i-Azam University, Pakistan. 1993.
Athar SM. Final report on Salmonella in poultry, poultry products, poultry feed, and feed ingredients. Directorate of poultry production and Research Sind. Karachi. Pakistan. 1982.
Javed T. Prevalence and pathological studies on salmonellosis in broiler breeders. Ph.D Thesis, Department of Biological Sciences, Quaid-i-Azam University. Islamabad. Pakistan. 1992.
Verma JC, Gupta BR. Determination of minimum inhibitory concentration of antibiotics against Salmonella organisms from animal sources by using multiple inoculation. Indian J Ani Sci 1992;62:793-6.
Maiorini E, Lopez EE, Marrow AL, Ramirez F, Procapio A. Fuvmanski, S et al. Multiple resistant non-typhoidal Salmonella gastroenteritis in children. Ped Infect Dis 1993;12:139-144.
Sajid SD. Epidemiology and molecular characterization of indigenous Salmonella isolates from poultry. Ph.D. Thesis. Deparment of Biological Sciences Quaid-i-Azam University Islamabad. 1998.
Ward LR, Threlfall EJ, Row B. Multiple drug resistance in Salmonella in England and Wales: a comparison between 1981 and 1988. J Clin Path 1990;43:563-6.
Son Ansary R, Salmah I, Maznah A. Survey of plasmids resistance factors among veterinary isolates of S .enteritidis in Malaysia. J Clin Path 1995;11:315-8.
Ward LR, Threlfall EJ. Human salmonellosis in England and Wales. Current situation. Symposium “Salmonella and salmonellosis” 20-22 May 1997. Plaufragan/Saint Brieve, France. Proceedings 1997; 8: 547-9.
Ling JM, Koo IC. Kam KM, Cheng AF. Antimicrobial susceptibility and molecular epidemiology of Salmonella enterica serotype Enteritidis isolates in Hong Kong from 1986-1996. J Clin Microbiol 1998;36:1693-9.
Olsen JE, Brown DJ, Baggessen DL, Bisgaard M. Biochemical and molecular characterization of Salmonella enterica serotype Berta and comparison of methods for typing. Epidemiol Infect 1992;108:243-60.
Threlfall EJ, Frost JA. Plasmid profile typing can be used to subdivide phage type 49 of Salmonella typhimurium outbreak investigation. Epidemiol Infect 1990;104: 243-51.