• Mehdi Mahmoodi
  • Mohammareza Hajizadeh
  • Hamidreza Rashidinejad
  • Gholamreza Asadikaram
  • Mohammad Khaksari
  • Mohammadreza Mirzaee
  • Naser Seyedi
  • Amir Rahnema
  • Ahmadreza Sayadi


Background: Complete blood count (CBC) is one of the most common and conventional bloodtest that physicians usually request. However the results of this test are affected by differentfactors such as, the temperature and duration of incubation, therefore the aim of this survey was toevaluate the effect of temperature and time of incubation on CBC, red blood cells (RBC) indicesand white blood cells (WBC) differential count. Methods: In a cross-sectional study, bloodsamples were taken from 30 healthy medical students of Rafsanjan University (15 males and 15females). The samples divided into three parts; CBC were done on the samples up to 48 hoursincubation at temperature of 25, 30, and 370 C at the time of sampling, and after 2, 8, 24 and 48hours. Data were statistically analyzed and the following results were obtained. Results: RBCcount, hematocrit, MCH, percent of monocytes and eosinophils were constant in differenttemperatures, WBC count, MCHC, hemoglobin, platelets count, the percent of lymphocytes andneutrophils were constant up to 24 hours and then tend to increase with increasing temperatureexcept lymphocytes percent that tend to decrease. MCV decreased with increasing temperature upto 8 hours and then significantly increased (from 83.89 to 87.50 fmol/l, p<0.001). WBC,hematocrit, MCV, platelets count, and neutrophils’ percent tend to increase by the time ofincubation, but RBC count, MCHC, lymphocytes’ percent decreased. Hemoglobin, MCH, and thepercent of monocytes and eosinophils were constant. Conclusion: The finding of this surveyshowed that some of CBC parameters can be changed with the incubation, therefore it is better todo the CBC test after blood taking as soon as possible.Key Words: CBC, RBC Indices, Temperature of storage, Time of storage


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