SURVEY OF CHANGES IN COMPLETE BLOOD COUNT AND RED CELL INDICES OF WHOLE BLOOD INCUBATED IN VITRO AT DIFFERENT TEMPERATURES UP TO 48 HOURS
Abstract
Background: Complete blood count (CBC) is one of the most common and conventional bloodtest that physicians usually request. However the results of this test are affected by differentfactors such as, the temperature and duration of incubation, therefore the aim of this survey was toevaluate the effect of temperature and time of incubation on CBC, red blood cells (RBC) indicesand white blood cells (WBC) differential count. Methods: In a cross-sectional study, bloodsamples were taken from 30 healthy medical students of Rafsanjan University (15 males and 15females). The samples divided into three parts; CBC were done on the samples up to 48 hoursincubation at temperature of 25, 30, and 370 C at the time of sampling, and after 2, 8, 24 and 48hours. Data were statistically analyzed and the following results were obtained. Results: RBCcount, hematocrit, MCH, percent of monocytes and eosinophils were constant in differenttemperatures, WBC count, MCHC, hemoglobin, platelets count, the percent of lymphocytes andneutrophils were constant up to 24 hours and then tend to increase with increasing temperatureexcept lymphocytes percent that tend to decrease. MCV decreased with increasing temperature upto 8 hours and then significantly increased (from 83.89 to 87.50 fmol/l, p<0.001). WBC,hematocrit, MCV, platelets count, and neutrophils’ percent tend to increase by the time ofincubation, but RBC count, MCHC, lymphocytes’ percent decreased. Hemoglobin, MCH, and thepercent of monocytes and eosinophils were constant. Conclusion: The finding of this surveyshowed that some of CBC parameters can be changed with the incubation, therefore it is better todo the CBC test after blood taking as soon as possible.Key Words: CBC, RBC Indices, Temperature of storage, Time of storageReferences
Hogman CF, Knutson I. Storage of whole blood before
separation: the effect of temperature on red cell 2,3-DPG and
the accumulation of lactate. Transfusion 1999; 39(5): 422-7
Gulati GL, Hyland LJ. Changes in automated complete blood
cell and differential leukocyte count results induced by
storage of blood at room temperature. Arch patted lab Med
; 126(3): 336-42
Shimizut L, Ishikawa Y. Biochemical and membrane
functional alterations in red cell during preparation and
storage of leukocytes and platelets: poor red cell suspensions
prepared by warm centrifuge method. Tohoku J Ex Med
; 145(3): 321-8
Knuston I, Loof H. Pre-separation storage of whole blood:
the effect of temperature on red cell 2,3-DPG and
myeloperoxidase in plasma, Transfusion 1999; 21(2): 111-15
Vogelaar SA, Posthuma D, Boomsma D, Kluft C. Blood
sample stability at room temperature for counting red and
white blood cells and platelets. Vascul Pharmacol 2002;
(3): 123-5
Hirase Y, Makatsuka H, Kawai T, Horiguchi S, Ikeda M.
Stable blood cell counts after one-week storage at room
temperature., Bull Environ Contam Toxicol 1992; 49(4):
-8
Wood BL, Andrews J, Miller S, Sabath DE. Refrigerated
storage improves the stability of the complete blood cell
count and automated differential. Am J Clin Pathol 1999;
(5): 687-95
Zhang JN, Wood J, Bergeron AL, McBride L, Ball C, Yu Q,
Pusiteri AE, Holcomb JB, Dong JF. Effects of low
temperature on shear-induced platelet aggregation and
activation. J Trauma 2004; 57(2): 216-23
Qi R, Yatomi Y, Ozaki Y. Effects of incubation time,
temperature, and anticoagulants on platelet aggregation in
whole blood. Thromb Res 2001; 101(3): 139-44
Shortland AP, Rhodes NP, Rattray A, Black RA, Williams
DF. The effect of temperature and shear rate on platelet
aggregation. Mater Sci Mater Med 1997; 8(12): 887-90
Uchida K, Sakai K, Ito E, Kwon OH, Kikuchi A, Yamato M,
Okano T. Temperature-dependent modulation of blood
platelet movement and morphology on poly(Nisopropylacrylamide)-grafted surfaces. Biomaterials 2000;
(9): 923-9
Ho CH, Chan IH. The influence of time of storage,
temperature of storage, platelet number in platelet-rich
plasma, packed cell, mean platelet volume, hemoglobin
concentration, age, and sex on platelet aggregation test. Ann
Hematol 1995; 71(3): 129-33.
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