EFFECT OF CIPROFOXACIN ON GROWING CARTILAGE IN ALBINO RAT PUPS

Authors

  • Haji Muhammad Aslam Channa
  • Muhammad Ashfaq
  • Shah Murad Mastoi
  • Muhammad Azam Qureshi

Abstract

Background: Administration of quinolone therapy is controversial during growing age as statedby earlier worker. The flroquinolones are currently not indicated for young children, because ofarthropathy and adverse effect on growing cartilage shown by studies. However the effects ofciprofloxacin on epiphyseal growth plate has remained undocumented. This study is therefore,undertaken to determine the risk of ciprofloxacin administration an growing cartilage byprospective experimental animal study model using Wistar albino rat pups. Methods:Ciprofloxacin was administered to newly born Wistar albino rat pups with a doze of 20mg/kgbody weight intraperitonealy twice a day from day-1 to day-14 after birth. The animals weresacrificed by deep ether anesthesia. The limbs were disarticulated from axial skeleton, soft tissuewas removed. The intact bone mean length in millimeter of right and left humerus and femur wasmeasured with the help of electronic vernier caliper and bones were fixed in 10% bufferedfarmalin. Decalcification was done in 10% nitric acid and 10% formic acid changes. Afterparaplast embeding, 4 mm thick longitudinal sections of the proximal long bones were cut by arotary microtome. Routine staining with haemotoxylin and eosin was performed.Histomorphometry was done measuring the thickness of epiphyseal cartilage and was comparedwith similar value of control animals. The results were statistically analysed to find out thesignificance. Results: The ciprofloxacin induces a mordanting effect as abviated by increasedbasophilia. Our study reveales that cirprofloxacin administration in the newly born pups decreasedthe width of epiphyseal growth plate cartilage by 10.43% in humerus and 4.72% in femur ascompared to the growth of control cartilage. The decrease in the width was brought about mainlyby the reduced count of the proliferative cells in the proliferative zone and the diminuation in theaverage size of the hypertrophic condryocytes in the hypertrophic zone. The reserve zone hasbecome markedly reduced in thickness. Conclusion: The ciprofloxacin post-natal administrationeffected growth plate retardation by inhibiting the mitosis in the proliferative zone and alsoeffected the mean length of humora & femora leading to reduction in limb length of rat pups.Key words: Ciprofloxacin – Cartilage Toxicity- Cartilage growth – Bone Ossification – Growthrate – Epiphyseal plate – Chondrocyte.

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