PLASTINATION OF WHOLE BRAIN SPECIMEN AND BRAIN SLICES
AbstractBackground: The human dead body specimens are plastinated for teaching purposes in medical institutions, usingsilicone. The silicone impregnated whole brain specimens and brain slices do not give satisfactory results. Methods:In the present study the brain specimens were plastinated with another polymer known as Polyester-Copolymer. Thebrain specimens were first preserved and then fixed with 5% formalin. The specimens were then dehydrated anddegreased in a volatile solvent acetone. The specimens were placed in Polyester- Copolymer solution which penetratedthe brain tissue both intracellulary and intercelluraly. The specimens were then cured by gas method. Results: Thewhole brain specimens and brain slices plastinated with Polyester-Copolymer were dry, odorless, handy and durable.It also gives a clear visual contrast between grey and white matter in brain slices whereas the brain specimensplastinated with silicone are flexible and sticky. There was no color contrast between grey and white matter.Conclusion: The polyester impregnated brain specimens and slices are non-toxic and ideal for teaching purposes andexaminations. They require minimal aftercare. The whole organ serial sections of plastinated brain specimens willhelp 3- dimensional study of the normal brain and will improve the assessment of brain pathology.
Hagens G. Plastination technique. Heidel. Plast. Folder. l986;
Brickley H C. Walker A N and Jackson R L and Donnars R S.
The preservation of pathology specimens by silicone
plastination An innovative adjunct to pathology education.
Am.J. Clin Pathol. 1987;88(2):220-23.
Hagens G. Impregnation of soft biological specimens with
thermosetting resins and elastomers. Anat. Rec. 1979; 194;
Klanes T and Wilhelm K. The current potential of plastination
Anat. Embr. 1987; 175:411-21.
Hagens G, Kriz. Plast. Folder. 1985;5-17.
Hagens and Tiedemann Plastination. Anat. Embr. 1984;210-
Schwab K H and Hagens G. Freeze substitution of
macroscopic specimens for plastination. Abstract. Sixth
European Anatomical Congress. Acta. Anat. 1981, 111(12)
Kieneke E W. Uhlmann K. Easy method of producing
biological specimens by molecular substitution. XII.
Int Anat Congr London. Abstr. 1985; 354.
Sittel C. Eckel H E and Sprinzl G M. Section plastination of
larynx for histology of whole organ sections. HNO. 1996;
Stoka K and Schit G. Utilization of the postmortem
examination with emphasis on audiovisual aids. Arch Pathol.
Lab Med. 1987;11 U9):883-84.
Hawley DA. Marlin DC, Cook DC, Becsay D, Clark M A,
Pless J I; and Stanish S M. Specimens for teaching forensic
pathology, odontology and anthropology. Am.J. ForensicMed. Pathol 1991; 12(2): 170-174.
Deegner P and Berndta W. Process of preserving animal
object. U.S. Pat.l914; l, 163,645.
Acheson E D Formaldehyde in British chemical industry.
Lancet. 1981; 1:611-616.
Daschner F. Formaldehyde. Klinikartz. 1983; 12(1): 9-10.
Pabst R Wetche Gefahren bestehen beim umgang mit.
Formaldehyde. Anat, Anz. 1985; 161:154.
Bickley H C. Plastination, a new technique for anatomic
pathology and forensic science. Pathol. Update Series.
Bickly H C, Hagens G and Townsend F M. An improved
method for preservation of teaching specimens. Arch. Pathol.
Lab. Med. 1981;105: 674-676.