PLASTINATION OF WHOLE BRAIN SPECIMEN AND BRAIN SLICES
Abstract
Background: The human dead body specimens are plastinated for teaching purposes in medical institutions, usingsilicone. The silicone impregnated whole brain specimens and brain slices do not give satisfactory results. Methods:In the present study the brain specimens were plastinated with another polymer known as Polyester-Copolymer. Thebrain specimens were first preserved and then fixed with 5% formalin. The specimens were then dehydrated anddegreased in a volatile solvent acetone. The specimens were placed in Polyester- Copolymer solution which penetratedthe brain tissue both intracellulary and intercelluraly. The specimens were then cured by gas method. Results: Thewhole brain specimens and brain slices plastinated with Polyester-Copolymer were dry, odorless, handy and durable.It also gives a clear visual contrast between grey and white matter in brain slices whereas the brain specimensplastinated with silicone are flexible and sticky. There was no color contrast between grey and white matter.Conclusion: The polyester impregnated brain specimens and slices are non-toxic and ideal for teaching purposes andexaminations. They require minimal aftercare. The whole organ serial sections of plastinated brain specimens willhelp 3- dimensional study of the normal brain and will improve the assessment of brain pathology.References
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