ANTI-HCV ANTIBODIES DETECTION-A COMPARISON BETWEEN METHODOLOGY: ELISA VS DIPSTICK ASSAY
AbstractHepatitis C accounts for 70-95% cases of post-transfusion hepatitis in different parts of the world. The high prevalenceof antibodies to hepatitis C virus (HCV) among frequently blood transfused patients (e.g. thalassaemic), those whoreceive blood products (e.g. haemophiliacs), and among intravenous drug abusers confirmed the parenteral route asthe major route of HCV infection. To promote screening of blood donors for HCV antibodies, a Dipstick HCV assaywas compared with third generation enzyme immunoassay. Fifty diagnosed cases of hepatitis C and twenty healthycontrol subjects were analyzed by both methods and hundred per cent same results were found.
Shah SHA. Abid S. Khan All. Chronic non-B,
non-C Hepatitis Report from Pakistan Am J
Gastroenterol 1992; 87:1061-62.
Wyngaarden .113. Smith LH. Bennett JC (eds).
Cecil Textbook of Medicine. 19lh lid
Philadelphia: VVB Saunders Company. 1992:
Ahmed M. Qureshi MS. Mushtaq S. Khan FA,
Ahmed SA. Zaheeruddin Acute sporadic hepatitis
non-A. non-B: Clinical features and biochemical
profile. JPMA 1989; 307-399.
Wang JEH A study on the epidemiology of
hepatitis C infection among blood donors in
Singapore. .1 Public Health Med 1995; 17: 387-
Bradley D. McCaustland K. Krawcznski K.
Spelbring .1. Humphrey C. Cook EH. Hepatitis C
virus: Buoyant density of the factor VIII derived
isolated in sucrose. .1 Med Virol 1991.34:206-8.
Choo Ql. Richman K. Han. JH. el al. Genetic
organization and diversity of the hepatitis C virus.
Proc Natl Acad Sci USA 1991:88:2451-55.
Malik IA. Ahmad N, Luqman M, el al. Hepatitis C
as a cause of chronic liver disease in northern
Pakistan. JPMA 1992: 67-68.
Weiner AJ. Kuo G. Bradley DW. et al. Detection
of hepatitis C viral sequences in non-A. non-B
hepatitis. Lancet 1990:335 1-3.
Malik IA. The prevalence, pattern and prevention
of viral hepatitis in Pakistan, souvenir 21s1
National PAP Conference, 1997: 66-67
Ramia S Hepatitis C virus: Molecular virology and
its implications for serologic diagnosis. Saudi .1
kidney Dis Transplant 1995:6(2) 190-96.
Al-Faleh I Z. Ramia S. Hepatitis C virus (HCV)
infection in Saudi Arabia: A review. Ann Saudi
Med 1997; 17:77- 82.
Van-del-Poel CL. Reesink II. Schaaberg W. el al.
infectivity of blood seropositive for hepatitis C
virus antibodies. Lancet 1990: 335:558-60.
Tremolada f. Casarim C, tagger A, et al. Antibody
to hepatitis C virus in post-transfusion hepatitis.
Ann Intern Med 1991; 114: 277-81
Fusconi M. I.enzi M. Ballardini G. et al Anti-I ICV
testing in autoimmune hepatitis and primary
biliary eirrhosisl. Lancet 1990: 336 823.
Mattsson I. Gutierrez R. Dawson GIL Lesniewski
RR, Mushahawar LK, Weiland O. antibodies to
recombinant and synthetic peptides derived from
the hepatitis C virus genome in long-term studied
patients with post-transfusion hepatitis C. Scand. J
Gastroenterol 1991:26: 1257-62.
Craxi A, Valenza M, Fabiano c. et al. Thirdgeneration hepatitis C virus tests in asymptomatic
anti-IIC’V positive blood donors. .1 Hepatol 1994;
Zauer HI.. Virelind H. Van Exel-Oehlers P.I. et al.
Confirmation of hepatitis C infection: A
comparison of five immunoblot assays,
Transfusion 1994; 34: 603-607.
Okamoto H. Tsuda f. Machida a. et al. Antibodies
against synthetic oliopeptides deduced from the
putative core gene for the diagnosis of hepatitis C
virus Hepatology 1992: 15: 180-86"
Nagavama R. Miyaakae K. Tsuda f. Okamoto H
IgM antibody to a hepatitis C virus core peptide
(CPU) for monitoring activity of liver disease in
patients with acute or chronic hepatitis C. J Med
Virol 1994; 42: 311-17.
Issar SK, rantakrishna BS, Ramakrishna B,
Christopher S. Samuel BU. Jhon T.I. Prevalence
and presentation of hepatitis C related chronic
liver disease in Southern India .1 Trap Med
Hygiene 1995: 98: 161-65.
Shimizu YK. Weiner AJ. Rosenblatt .1. et al. Early
events in hepatitis C’ virus infection of
chimpanzees. Proc Natl Acad Sei USA 1990; 87:
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