EVALUATION OF DIFFERENT TRANSPORT AND ENRICHMENT MEDIA FOR THE ISOLATION OF HELICOBACTER PYLORI
Abstract
Background: Helicobacter pylori are traditionally difficult to grow on culture media. The present study aims todetermine the optimal transport and culture media for growing the microorganisms. Methods: One hundred antralbiopsies were obtained in duplicate from patients complaining of upper GIT symptoms. Growth was obtained from44% biopsies transported in Semi Solid Motility Medium (SSMM) and 42% from thioglycolate broth. This differencewas not statistically significant (p = 0.l). Brain Heart Infusion Agar (BH1) plus 7% Horse Red Blood Cells (HRBC)plus SR69 (antibiotic supplement) was found to be the best of three culture media for isolation of H. pylori, with agrowth rate of 45% (p=<0.001). Results: This study shows that H. pylori is a common cause of gastro-duodenal diseasein Pakistan. Proper transport and selection of culture media is required for optimal isolation from gastric biopsiesReferences
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