• M. Hussain Khan
  • Syed Humayun Shah


Background: A small number of persons with Rh-negative blood have red blood cells that are not directly agglutinatedby anti D during routine testing for 'Rh' antigen, but that do react when the D-typing test is converted to the indirectantiglobulin test, which is Du' test. Cells thus identified are then designated as 'Du' cells. This study was done to assessthe incidence of ‘Du’ patients in Rh negative obstetrics patients. Methods: A total of 1500 pregnant women werescreened for Rh antigen. The red blood cells of the D negative subjects were then tested by indirect method.Agglutination confirmed 'Du’ otherwise it was confirmed as D (-). Results: Out of the 50 D?negative women subjectedto the indirect antiglobulin test 6 women were found to have Du’ blood. Conclusions: This reflects a significantpercentage of D' negative. Du' subjects in our obstetrics patients. Therefore, utmost care should be taken beforelabeling a subject D' negative.


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