• Bibi Hajra Women Medical College, Abbottabad
  • Shaukat Ali Orakzai Women Medical College, Abbottabad
  • Uzma Faryal Women Medical College, Abbottabad
  • Mukhtar Hassan Hazara University, Mansehra
  • Shazia Rasheed CMH Lahore Medical College, Lahore
  • Salim Wazir Ayub Medical College, Abbottabad


Background: Diabetes mellitus constitutes one of the most important problems in developing and non-developing countries. The purpose of the study to estimate the concentrations of Chromium and Manganese in diabetic and non-diabetic population of Hazara division. The cross sectional comparative study was carried out on one hundred blood samples of Type 2 Diabetic patients collected non-randomly from Ayub Teaching Hospital and one hundred normal healthy controls from Women Medical College Abbottabad from September 2014 to April 2015. Methods: The study included two hundred subjects. Among them 100 were diabetic and 100 non diabetic respectively. The blood samples were collected from Ayub Medical College, Abbottabad. The serum Chromium and Manganese levels were determined by Atomic Absorption spectrophotometer. Results: Serum Chromium and Manganese levels were decreased in diabetic and increased in non-diabetic patients. Conclusion: Low serum level of Chromium and manganese were found in diabetic patients as compare to non-diabetic individuals.Keywords: .Chromium; Manganese Atomic Absorption Spectroscopy

Author Biography

Bibi Hajra, Women Medical College, Abbottabad

Lecturer, Biochemistry department


Albert KG. Zimmet PZ. Definition diagnosis and classification of diabetes mellitus and its complication diagnosis and classification provisional report of WHO consultation. Diabet Med 1998;15(7):539–53.

Ozougwu J, Obimba K, Belonwu C, Unakalamba C. The pathogenesis and pathophysiology of type 1 and type 2 diabetes mellitus. J Physiol Pathophysiol 2013;4(4):46–57.

Weyer C, Bogardos C, Mott DM, Prately RE. The natural history of insulin secretory dysfunction and insulin resistance in the pathogenesis of type 2 diabetes mellitus. J Clin Invest 1999;104(6):787–94.

Vionnet N, Stoffel M, Takeda J, Yasuda K, Bell GI, Zouali H,

et al. Nonsense mutation in the glucokinase gene causes early-onset noninsulin-dependent diabetes mellitus. Nature 1992;356(6371):721–2.

Humayun M, Khalid A, Ali A. To Study the Levels of Serum Chromium, Copper, Magnesium and Zinc in patients with Diabetes Mellitus Type 2. [internet]. [cited 2015 Aug 20]. Available from:

King H, Aubert RE, Herman WH: Global burden of diabetes, 1995–2025: prevalence, numerical estimates, and projections. Diabetes Care 1998;21(9):1414–31.

Puri M,Gujral U,Nayyar SB.Compartive study of serum Zn,Mg and Cu levels among patients of type 2 diabetes with without microangliopathic complications.IJMHS

Fairweather D, Rose NR. Type 1 Diabetes: Virus infection or autoimmune disease? Nature Immunology 2002;3(4):338–40.

Tuman RW, Doisy RJ. Metabolic effects of the glucose tolerance factor (GTF) in normal and genetically diabetes mice. Diabetes 1977;26(9):820–6.

Trow LG, Lewis J, Greenwood RH, Sampson MJ, Self KA, Crews HM, et al. Lack effect of dietary Chromium supplementation on glucose tolerance, plasma insulin and lipoprotein level in patient with type 2 diabetes. Int J vitam Nutr Res 2000;70(1):14–8.

Koh ES, Kim SJ, Yoon HE, Chung JH, Chung S, Park CW, et al, Association of blood manganese level with diabetes and renal dysfunction: a cross-sectional study of the Korean general population. BMC Endocr Disord 2014;14:24.

Baynes JW, Dominiczak MH, editors. Medical Biochemistry, 3rd ed, London: Mosby Elsevier. 2009. p. 286–7.

Elabid BH,Ahmed AM. Serum Chromium, Manganese, Zinc and Hemoglobin A 1c % in Sudanese with Type 2 Diabetes. Life Sci J 2014;11(9):320–2.

Anderson RA, Cheng N, Bryden NA, Polansky MM, Cheng N, Chi J, et al. Elevated Intakes of Supplemental Chromium Improve Glucose and Insulin Variables in Individuals With Type 2 Diabetes. Diabetes 1997;46(11):1786–91.

Kazi TG, Afridi HI, Kazi N, Jamali MK, Arain MB, Jalbani N, et al. Copper, chromium, manganese, iron, nickel, and zinc levels in biological samples of diabetes mellitus patients. Biol Trace Elem Res 2008;122(1):1–18.

Ekin S, Mert N, Gunduz H, Meral I. Serum sialic acid levels and selected mineral status in patients with type 2 diabetes mellitus. Biol Trace Elem Res 2003;94(3):193–201.

Forte G, Bocca B, Peruzzu A, Tolu F, Asara Y, Farace C, et al. Blood metals concentration in type 1 and type 2 diabetics. Biol Trace Elem Res 2013;156(1-3):79–90.

Hasan HG, Ismael PA, Aziz NM. Evaluation of Serum Chromium Levels in Patients with Type1 and 2 Diabetes Mellitus and insulin resistance. Int J Basic App Sci 2012;12(4):69–73.

Walter RM, Uriu-Hare JY, Olin KL, Oster MH, Anawalt BD, Critchfield JW, et al. Copper, zinc, manganese and magnesium status and complications of diabetes mellitus. Diabetes Care 1991;14(11):1050–6.

Nicoloff G, Mutafchiev K, StrashimirovD, Petrova C. Serum manganese in children with diabetes mellitus type 1. Diabetol Croat 2004;33(2):47–51.

Bocca B, Madeddu R, Asara Y, Tolu P, Marchal JA, Forte G. Assessment of reference ranges for blood Cu, Mn, Se and Zn in a Se and Zn in a selected Italian population. J Trace Elem Med Biol 2011;25(1):19–26.

Clark NA, Teschke K, Rideout K, Copes R. Trace element levels in adults from the west coast of Canada and associations with age, gender, diet, activities, ad levels of other trace elements. Chemosphere 2007;70(1):155–64.

Morris BW, MacNeil S, Hardisty CA, Heller S, Burgin C, Gray TA. Cr homeostasis in patient with tpe 2 NIDDM diabetes. J Trace Elem Med Biol 1999;13(1-2):57–61.

Farid SM. The Association between serum glucose and serum Lead and Selected Trace Elements in Type 2 Diabetes mellitus Patients in Jeddah, Saudi Arabia. Med J Islam World Acad Sci 2012;20(4):130–9.

Zhao C, Wang H, Zhang J, Feng L. Correlations of trace elements, glucose and body compositions in type 2 diabetics.Wei Sheng Yan Jiu 2008;37(5):600–5.




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