IMPACT OF PRENATAL ADMINISTRATION OF MELAMINE ON FOETAL GROWTH IN RATS
AbstractBackground: Data on the potential effects of maternal exposure to melamine is scarce. We aimed to evaluate the impact of melamine administration on pregnancy outcome and foetal growth in rats. Methods: Positively-mated female Sprague-Dawley rats (n=24) were treated from day 6to day 20of gestation with vehicle (control), melamine 300 mg/kg/day (group-1) or melamine 450 mg/kg/day (group 2). On day 21, the numbers of foetal resorptions and dead foetuses were recorded. Thereafter, pups were examined for external anomalies, and various growth parameters were measured. Results: A remarkable increase in the number of resorptions was observed in group-2 compared to the other two groups. A significant increase in foetal weight and placental weight was seen in group-2 compared to control. Head length and placental diameter were low in group-1 compared to control. The ratio between crown-rump length and head length was significantly greater in group 2 compared to control indicating asymmetrical intrauterine growth restriction. The only influence observed in group 1 compared to control was a decrease in placental diameter. No gross foetal malformations or changes in umbilical cord length, crown-rump length or biparietal diameter were observed in both melamine-treated groups. Conclusions: Maternal exposure to melamine during pregnancy increased the incidence of resorption and resulted in asymmetrical intrauterine growth restriction. Keywords: Melamine; Maternal Exposure; Foetal Growth; Rat
Brown CA, Jeong KS, Poppenga RH, Puschner B, Miller DM, Ellis AE, et al. Outbreaks of renal failure associated with melamine and cyanuric acid in dogs and cats in 2004 and 2007. J Vet Diagn Invest 2007;19(5):525–31.
Skinner CG, Thomas JD, Osterloh JD. Melamine toxicity. J Med Toxicol 2010;6(1):50–5.
Chiu MC. Melamine-tainted milk product (MTMP) renal stone outbreak in humans. Hong Kong Med J 2008;14(6):424–6.
Wang Z, Luo H, Tu W, Yang H, Wong WH, Wong WT, et al. Melamine-tainted milk product-associated urinary stones in children. Pediatr Int 2011;53(4):489–96.
Cao Y, Yi ZW, Zhang H, Dang XQ, Wu XC, Huang AW. Etiology and outcomes of acute kidney injury in Chinese children: a prospective multicentre investigation. BMC Urol 2013;13:41.
Wen JG, Liu XJ, Wang ZM, Li TF, Wahlqvist ML. Melamine-contaminated milk formula and its impact on children. Asia Pac J Clin Nutr 2016;25(4):697–705.
Puschner B, Reimschuessel R. Toxicosis caused by melamine and cyanuric acid in dogs and cats: uncovering the mystery and subsequent global implications. Clin Lab Med 2011;31(1):181–99.
Hau AK, Kwan TH, Li PK. Melamine toxicity and the kidney. J Am Soc Nephrol 2009;20(2):245–50.
Hu P, Wang J, Zhang M, Hu B, Lu L, Zhang CR, et al. Liver involvement in melamine-associated nephrolithiasis. Arch Iran Med 2012;15(4):247–8.
An L, Sun W. A Brief Review of Neurotoxicity Induced by Melamine. Neurotox Res 2017;32(2):301–9.
Ogasawara H, Imaida K, Ishiwata H, Toyoda K, Kawanishi T, Uneyama C, et al. Urinary bladder carcinogenesis induced by melamine in F344 male rats: correlation between carcinogenicity and urolith formation. Carcinogenesis 1995;16(11):2773–7.
Wong WT, Tian XY, Lau CW, Wang YX, Liu J, Cheang WS, et al. Renal and vascular function in pregnant and neonatal rats exposed to melamine and related compounds. Hong Kong Med J 2013;19(Suppl 8):31–3.
Jingbin W, Ndong M, Kai H, Matsuno K, Kayama F. Placental transfer of melamine and its effects on rat dams and foetuses. Food Chem Toxicol 2010;48(7):1791–5.
El-Nezami H, Tam PK, Chan Y, Lau AS, Leung FC, Chen SF, et al. Impact of melamine-tainted milk on foetal kidneys and disease development later in life. Hong Kong Med J 2013;19(Suppl 8):34–8.
Wang CC, Fung KP, Fok TF, Lau TK, Pang CP, Chu KO, et al. Melamine toxicity in rat foetuses and infants. Hong Kong Med J 2013;19(Suppl 8):20–2.
Gamboa da Costa G, Jacob CC, Von Tungeln LS, Hasbrouck NR, Olson GR, Hattan DG, et al. Dose-response assessment of nephrotoxicity from a twenty-eight-day combined-exposure to melamine and cyanuric acid in F344 rats. Toxicol Appl Pharmacol 2012;262(2):99–106.
Reimschuessel R, Puschner B. Melamine toxicity--stones vs. crystals. J Med Toxicol 2010;6(4):468–9.
Gao J, Wang F, Kuang X, Chen R, Rao J, Wang B, et al. Assessment of chronic renal injury from melamine-associated pediatric urolithiasis: an eighteen-month prospective cohort study. Ann Saudi Med 2016;36(4):252–7.