ANTIOXIDANT EFFECT OF VITAMIN E ON CARBON TETRACHLORIDE INDUCED TUBULOINTERSTITIAL AND GLOMERULAR DAMAGE IN THE KIDNEYS OF ALBINO MICE
AbstractBackground: Chemical induced nephrotoxicity is one of the main causes of acute kidney injury. The objective of this study was to determine the antioxidant effect of vitamin E against carbon tetrachloride induced tubulointerstitial and glomerular damage in the kidney of albino mice. Methods: The study had been conducted on albino mice. The duration of study was for five weeks. A total of 35 animals were randomly divided into five groups A, B, C, D and E .The group A served as control group, group B was administered only with carbon tetrachloride (no vitamin E) and groups C, D and E received test drug (vitamin E) in doses of 1, 10 and 50mg/kg body weight respectively along with CCl4. The animals were dissected and kidneys were excised for microscopic study for possible histo-morphological effects. Results: It was observed that carbon tetrachloride treated experimental groups developed tubulo-interstitial and glomerular changes as compared to control group A. The results suggested that these changes were significantly reduced in vitamin E treated groups especially in dose of 50 mg/kg body weight. Conclusion: This study reveals that tubulo-interstitial and glomerular damage caused by carbon tetrachloride can be reduced by vitamin E in dose of 50 mg/kg body weight.Keywords: Vitamin E; Carbon tetrachloride and Glomerular damage
Shahbazi F, Dashti-Khavidaki S, Khalili H, Lessan-Pezeshki M. Potential renoprotective effects of silymarin against nephrotoxic drugs: a review of literature. J Pharm Pharm Sci 2012;15(1):112–23.
Hoitsma AJ, Wetzels JF, Koene RA. Drug induced nephrotoxicity. Aetiology, clinical features and management, Drug Saf 1991;6(2):131–47.
Barry M, Brenner FC. Rector. The kidney 6th ed. Vol I, Philadelphia W.B. Saunders, 2000; p.3–67.
Ogeturk M, Kus I, Colakoglu N, Zararsiz I, Ilhan N, Sarsilmaz M. Caffeic acid phenethyl ester protects kidneys against carbon tetrachloride toxicity in rats. J Ethnopharmacol 2005;97(2):273–80.
Ohta Y, Kongo M, Sasaki E, Nishida K, Ishiguro I. Therapeutic effect of melatonin on carbon tetrachloride‐induced acute liver injury in rats. J Pineal Res 2000;28(2):119–26.
Bhattacharya H, Lun L, Gomez GD. Biochemical effects to toxicity of CCL4 on rosy barbs (Puntius conchonius) Our Nat 2005;3(1):20–5.
Khan MR, Rizvi W, Khan GN, Khan RA, Shaheen S. Carbon tetrachloride-induced nephrotoxicity in rats: Protective role of Digera muricata. J Ethnopharmacol 2009;122(1):91–9.
Sreelatha S, Padma PR, Umadevi M. Protective effects of Coriandrum sativum extracts on carbon tetrachloride-induced hepatotoxicity in rats. Food Chem Toxicol 2009;47(4):702–8.
Adaramoye O. Comparative effects of vitamin E and kolaviron (a biflavonoid from Garcinia kola) on carbon tetrachloride-induced renal oxidative damage in mice. Pak J Biol Sci 2009;12(16):1146–51.
Venkatanarayana G, Sudhakara G, Sivajyothi P, Indira P. Protective effects of curcumin and vitamin E on carbon tetrachloride-induced nephrotoxicity in rats. EXCLI J 2012;11:641–650.
El-kholy TA, Naglaa H, Abbas HY. Protection of the Mushroom (shiitake" Lentinus-edodes) against Carbon-Tetrachloride-Induced Renal Injury in Rats. Life Sci J 2013;10(1):1701–8.
Paller MS. Drug induced nephropathies. Med Clin North Am1990;74(4):909–17.
Khan RA, Khan MR, Sahreen S. CCl 4-induced hepatotoxicity: protective effect of rutin on p53, CYP2E1 and the antioxidative status in rat. BMC Complement Altern Med 2012;12(1):178.
Chavez R, Fraser DJ, Bowen T, Jenkins RH, Nesargikar P, Pino-Chavez G, et al. Kidney ischaemia reperfusion injury in the rat: the EGTI scoring system as a valid and reliable tool for histological assessment. J Histol Histopathol 2016;3(1):1–7.
Ganie SA, Haq E, Hamid A, Qurishi Y, Mahmood Z, Zargar BA, et al. Carbon tetrachloride induced kidney and lung tissue damages and antioxidant activities of the aqueous rhizome extract of Podophyllum hexandrum. BMC Complement Altern Med 2011;11(1):17.
Manjrekar AP, Jisha V, Bag PP, Adhikary B, Pai MM, Hegde A, et al. Effect of Phyllanthus niruri Linn treatment on liver, kidney and testes in CCl 4 induced hepatotoxic rats. Indian J Exp Biol 2008;46(7):514–20.
Burk RF, Lane JM, Patel K. Relationship of oxygen and glutathione in protection against carbon tetrachloride-induced hepatic microsomal lipid peroxidation and covalent binding in the rat. Rationale for the use of hyperbaric oxygen to treat carbon tetrachloride ingestion. J Clin Invest 1984;74(6):1996–2001.
Ozturk F, Ucar M, Ozturk IC, Vardi N, Batcioglu K. Carbon tetrachloride-induced nephrotoxicity and protective effect of betaine in Sprague-Dawley rats. Urology 2003;62(2):353–6.
Ozden S, Catalgol B, Gezginci-Oktayoglu S, Karatug A, Bolkent S, Alpertunga B. Acute effects of methiocarb on oxidative damage and the protective effects of vitamin E and taurine in the liver and kidney of Wistar rats. Toxicol Ind Health 2013;29(1):60–71.