EXPRESSION OF PEROXISOME-PROLIFERATOR ACTIVATED RECEPTORS- IN DIABETICS, OBESE AND NORMAL SUBJECTS

Authors

  • Naeema Afzal Department of Pathology, Ayub Medical College, Abbottabad
  • Mukhtiar Hassan Faculty of Health Sciences, Hazara University
  • Sadia Fatima Department of Biochemistry, Ayub Medical College, Abbottabad
  • Sumbal Tariq Department of Pharmacology, Ayub Medical College, Abbottabad
  • Iftikhar Qayum Director Medical Research, Rehman Medical College, Peshawar

Abstract

Background: Current research in type 2 diabetes mellitus focuses on the role of Peroxisome-Proliferator Activated Receptors (PPARs) in the pathogenesis of the Insulin Resistance Syndrome (IRS), which are pre-diabetic lesion and the hallmark of fully developed type 2 diabetes mellitus. This study aims at identifying the abnormal status of the PPAR-g in adipose tissues of type 2 diabetes mellitus patients, when compared with matched normal controls. Methods: This cross-sectional study was conducted in Ayub Medical College, Abbottabad, from 2012 to 2014. Sample included three equal groups of patients. Group-1 with diagnosed type 2 diabetes mellitus, aged 40–65 years, acting as the test group, Group-2 included non-diabetic obese, and Group-3 with normal subjects. Transcription Factor Assay for Peroxisome Proliferator Activated Receptor Gamma (gamma PPAR) was done on ELISA Technique from Nuclear Extract procured from Adipose Tissue of the subjects. Results: Mean age of enrolled participants was 48.93 SD±6.52.years. Patients ranged between ages of 40 years to 67 years. The mean values of PPAR in normal, obese and diabetic group were 1.72 SD±0.28, 1.282 SE±0.18 and 1.283 SE±0.18 respectively. The difference in mean values of PPAR was significant p<0.05. Conclusion: The levels of PPAR-g in patients with type 2 Diabetes Mellitus and Obese cases are significantly lower than normal controls.

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Published

2016-03-10

Issue

Vol. 28 No. 1 (2016): JOURNAL OF AYUB MEDICAL COLLEGE, ABBOTTABAD

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ORIGINAL ARTICLE

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