EFFECT OF MELATONIN ON SERUM GLUCOSE AND BODY WEIGHTS IN STREPTOZOTOCIN INDUCED DIABETES IN ALBINO RATS
AbstractBackground: It has been demonstrated in experimental animal models that oxidative stress causes persistent and chronic hyperglycaemia, causing reduction in antioxidant defence system, ultimately leading to accumulation of free radicals.This study was performed to observe the effect of melatonin on serum glucose and body weights in streptozotocin induced diabetes in albino rats. Methods: Forty healthy adult male albino rats were included in the study and divided equally into 4 groups for 6 weeks. Group-A was taken as control. Group-B received streptozotocin I/P in a dose of 37 mg/kg body weight. Group-C received 10 mg/100 ml melatonin in drinking water and Group-D received only melatonin. Results: Streptozotocin significantly increased serum glucose and decreased weight in group B animals, whereas in group C, melatonin significantly restored serum glucose but could not restore the body weights reduced by streptozotocin. There was a significant reduction in body weight in melatonin treated group D animals. Conclusion: Melatonin decreases oxidative stress and hyperglycemia, but cannot restore the body weight reduced by streptozotocin. In fact, it further reduces body weight both in diabetic and normal state.Keywords: Streptozotocin, Melatonin, Oxidative stress, Reactive Oxygen Species, Diabetes Mellitus, Hyperglycaemia, Albino rats
Ceriello A. Oxidative stress and glycaemic regulation. Metabolism 2000;49:27–9.
Bhor VM, Raghuram N, Sivakami S. Oxidative damage and altered antioxidant enzyme activities in the small intestine of streptozotocininduced diabetic rats. Int J Biochem Cell Biol 2004;36:89–97.
Sharma VK. ‘Streptozotocin: an experimental tool in diabetes and Alzhiemers Disease’. Int J Pharm Res Development 2009;2(1):1–7.
Raza H, John A. Streptozotocin-induced cytotoxicity, oxidative stress and mitochondrial dysfunction in human hepatoma HepG2 cells. Int J Mol Sci 2012;13(5):5751–67.
Bolzan AD, Bianchi MS, Genotoxicity of streptozotocin. Mutat Res 2002;512:121–34.
Baydas G, Canatan H, Turkoglu A, Comparative analysis of the protective effects of melatonin and vitamin E on streptozotocin-induced diabetes mellitus. J Pineal Res 2002:32;225–30.
Renno WM, Abdeen, S, Alkhalaf, M, Asfer, S. Effect of green tea on kidney tubules of diabetic rats. Br J Nutr1 2008:100(3);652–9.
Ordóñez P, Moreno M, Alonso A, Fernández R, Díaz F, González C. ‘Insulin sensitivity in streptozotocin-induced diabetic rats treated with different doses of 17 β-oestradiol or progesterone. Exp Physiol 2007;92:241–9.
Derlacz RA, Poplawski, P, Napierala, M, Jagielski AK, Bryla J. Melatonin-induced modulation of glucose metabolism in primary cultures of rabbit kidney-cortex tubules. J Pineal Res 2005:38;164–9.
Quiroz Y, Ferrebuz A, Romero F, Vaziri ND, Rodriguez-Iturbe B. Melatonin ameliorates oxidative stress, inflammation, proteinuria and progression of renal damage in rats with renal mass reduction. Am J Physiol Renal Physiol 2008;294(2):F336–44.
Hardeland R. Anti-oxidative protection by melatonin: multiplicity of mechanisms from radical detoxification to radical avoidance. Endocrine 2005;27(2):119–30.
Anwar MM, Meki, AR. Oxidative stress in streptozotocin-induced diabetic rats: effects of garlic oil and melatonin. Comp Biochem Physiol Mol Integr Physiol 2003;135:539–47.
Tan DX, Manchester LC, Reiter RJ, Qi WB, Karbownik M, Calvo JR. Significance of melatonin in anti-oxidative defence system: reactions and products. Biol Signals Receipt 2000;9(3-4):137–59.
Hidayat M, ‘Protective Role Of Melatonin and Insulin On Streptozotocin Induced Nephrotoxicity in Albino Rats’, Pak. J. of Med.& Health Sci.(pjmhs) 07/2012; 6(3):.669-674..
Zafar M, Naqvi SN, Ahmed M, Kaimkhani ZA. Altered kidney morphology and enzymes in streptozotocin Induced Diabetic rats’, Int J Morphol 2009:27(3);783–90.
Oscika TM, Yu Y, Panagiotopoulos S, Clavant SP, Kirizis Z, Pike RN et al. Prevention of albuminuria by aminoguanidine or ramipril in streptozotocin-induced diabetic rats is associated with the normalization of glomerular protein kinase C. Diabetes 2000;49(1):87–93.
Anisimov VN, Zavarzina NY, Zabezhinski MA, Popovich IG, Zimina OA, Shtylick AV. Melatonin increases both life span and tumor incidence in female CBA mice. J Gerontol A Biol Sci Med Sci 2001;56:B311–23.
Bartness TJ, Demas GE, Song CK. Seasonal changes in adiposity: the roles of the photoperiod, melatonin and other hormones, and sympathetic nervous system. Exp Biol Med 2002;227:363–76.
Wolden-Hanson T, Mitton DR, McCants RL, Yellon SM, Wilkinson CW, Matsumoto AM, et al. Daily melatonin administration to middle-aged male rats suppresses body weight, intraabdominal adiposity, and plasma leptin and insulin independent of food intake and total body fat. Endocrinology 2000;141:487–97.
Costa EJ, Lopes RH, Lamy-Freud MT. Permeability of pure lipid bilayers to melatonin. J Pineal Res 1995;19:123–6.
Peschke E, Wolgast S, Bazwinsky I, Pönicke K, Muhlbauer E. Increased melatonin synthesis in pineal glands of rats in streptozotocin induced type 1 diabetes. J Pineal Res 2008;45(4):439–48.
Vural H, Sabuncu T, Arslan SO, Aksoy N. Melatonin inhibits lipid peroxidation and stimulates the antioxidant status of diabetic rats. J Pineal Res 2001;31:193–8.
Howarth FC, Jacobson M, Shafiullah M, Adeghate E. Long-term effects of streptozotocin-induced diabetes on the electrocardiogram, physical activity and body temperature in rats. Exp Physiol 2005;90:827–35.
Alonso-Vale MI, Andreotti S, Peres SB, Anhê GF, das Neves Borges-Silva C, Neto JC et al. Melatonin enhances leptin expression by rat adipocytes in the presence of insulin. Am J Physiol Endocrinol Metab 2005;288(4):805–12.
Tan DX, Manchester LC, Fuentes-Broto FL, Paredes SD, Reiter RJ. Significance and application of melatonin in the regulation of brown adipose tissue metabolism: relation to human obesity. Obes Rev 2011;12:167–88