EFFECT OF L-ARGININE AND INSULIN ON ADRENAL GLAND DAMAGED BY STREPTOZOTOCIN IN ALBINO RATS
AbstractBackground: The adrenal gland is one of the major endocrine glands involved not only in different physiological functions, but also in response to stress This study was done to asses the effects of L-arginine and insulin on streptozotocin (STZ) induced adrenal gland damage in albino rats. Method: This laboratory based experimental study on animals was undertaken in the Anatomy department of Basic Medical Sciences Institute (BMSI), Jinnah postgraduate Medical Center (JPMC), Karachi, from February to March 2018. Forty adult, healthy male albino rats were placed into 4 groups (10 each). Group A was taken as control. Group B was given STZ. Group C and D were given STZ as in group B with insulin and L-arginine respectively. Absolute and relative weight of adrenal glands was measured at the end of the study. Tissues from adrenal glands were processed and stained with Haematoxylin and Eosin for the morphometric study. Results: The absolute as well as relative adrenal weight of animals was significantly raised in group B in comparison to control, although showed a significant recovery in group C and D animals when insulin and L-arginine were added to STZ. Haematoxylin and Eosin (H&E) stained sections of adrenal cortex of STZ treated Group B showed reduced width of zona glomerulosa, with increased width of zona fasciculata and zona reticularis when compared to control. The width of these zones of adrenal cortex recovered to a significant extent when group C and D tissue sections were compared with STZ- treated group B tissue sections. Conclusion: This study highlighted the protective effect of L-arginine on adrenal gland weight and histology in streptozotocin induced adrenal gland damage, which was comparable to insulin. Keywords: Streptozotocin; L-arginine; Adrenal gland; Insulin
Mahar Y, Shoro AA, Naqvi A. The effect of L-Arginine and Insulin on Histological Changes in Streptozotocin treated Rat Adrenal Gland. PJMHS, 2012; 6 (4): 843-6.
Hany W, Malak A, Amin MA. Protective Role of L-arginine on the Suprarenal Gland of Adult Male Albino Rats Subjected to Recurrent Acute Restraint Stress: A Histological and Immunohistochemical Study. The Egyptian Journal of Anatomy, 2018; 41(1):156-71.
Chan O, Chan S, Inouye K, Vranic M, Matthews SG. Molecular Regulation of the Hypothalamo- Pituitary Adrenal Axis in Streptozotocin-Induced Diabetes: Effects of Insulin Treatment. Endocrinology, 2001; 142(11): 4872–9.
Gong S, Miao Y, Jiao G, Sun M, Li H, Lin J, et al. Dynamics and Correlation of Serum Cortisol and Corticosterone under Different Physiological or Stressful Conditions in Mice. PLoS One, 2015;10(2): e0117503. Published online 2015 Feb 20. DOI: 10.1371/journal.pone.0117503
Hwang SL, Liu IM, Tzeng TF, Cheng JT. Activation of imidazoline receptors in adrenal gland to lower plasma glucose in streptozotocin-induced diabetic rats. Diabetologia 2005; 48: 767–75. DOI 10.1007/s00125-005-1698-2
Mahar Y , Quddus I , Qureshi FM, Assad T, Qamar A. Restorative Effect of L-Arginine on Gross Morphology and Adrenal Weight in Streptozotocin Induced Diabetic Rats. JBUMDC 2017; 7(4): 218-22.
Sainz JM, Reche C, Rábano MA, Mondillo C, Patrignani ZJ, Macarulla JM, et al. Effects of Nitric Oxide on Aldosterone Synthesis and Nitric Oxide Synthase Activity in Glomerulosa Cells from Bovine Adrenal Gland. Endocrine, 2004; 24 (1): 61–71.
Elbassuoni EA, Ragy MM, Ahmed SM. Evidence of the protective effect of L-arginine and vitamin D against monosodium glutamate- induced liver and kidney dysfunction in rats. Biomedicine and Pharmacotherapy, 2018;108:799-808.
Cymeryng CB, Lotito SP, Colonna C, Finkielstein C,Pomeraniec Y, Grio´n N, et al. Expression of Nitric Oxide Synthases in Rat Adrenal Zona Fasciculata Cells. Endocrinology, 2002; 143(4):1235–42.
Repetto EM, Pannunzio V, Astort F, Calejman CM, Moreno MB, Pignataro OP, et al. Characterization of L-arginine transport in adrenal cells: effect of ACTH. Am J Physiol Endocrinol Metab, 2006; 291: 291–7.
Ahmad A, Dempsey SK, Daneva Z, Azam M, Li N, Li P, et al. Role of Nitric Oxide in the Cardiovascular and Renal Systems. International Journal of Molecular Sciences 2018; 19(9): 2605 https://doi.org/10.3390/ijms19092605
Heikkila M, Peltoketo H, Leppaluoto J, Ilves M, Vuolteenaho O, Vainio S.Wnt-4 Deficiency Alters Mouse Adrenal Cortex Function, Reducing Aldosterone Production.Endocrinology, 2002; 143 (11): 4358–65.
Hidayat M, Shoro AA, Naqvi A. Protective Role of Melatonin and Insulin in Streptozotocin induced Nephrotoxicity in Albino Rats. Pak J. Med Health Sci 2012; 6(3): 669 - 74.
Hidayat M, Nisar MF, Akram SF, Zahid MA. Effects of STZ on the morphology of proximal convoluted tubules in albino rats. Pak J. Med Health Sci 2012; 6(2): 298 -301.
Cherian L, Chacko G, Goodman C and Robertson CS. Neuroprotective effect of L-Arginine administration after cortical impact injury in rats: dose response and time window. Journal of Pharmacology and Experimental therapeutics, 2003;304:617-32.
Sohrabi D, Alipur M, Mellati AA. Effects of metronidazole on spermatogenesis, plasma gonadotrophin and testosterone in rats. Iranian J reprod Med. 2007; 5: 69-72.
Bancroft JD, Gamble M. Theory and practical of Histological Techniques. 6th ed. Edinburg: Elsevier Churchill Livingstone; 2008: 25-7,136-8.
Rosol TJ, Yarrington JT, Latendresse J, Capen CC. Toxicol Pathol 2001; 29(1): 41-8.
Zafar M, Naqvi SNH. Effect of STZ- Induced Diabetes on the Relative Weight of Kidney, Liver and Pancreas in Albino Rats; A Comparative Study. Int J Morphol 2010; 28 (1): 135-42.
Mahar Y, Waqar H, Salman S. Protective Effect of L-Arginine on Streptozotocin- Induced Diabetic Nephropathy in Albino Rat. JBUMDC 2017;7(1): 28-31.
Chan O, Inouy K, Akirav E, Park E, Riddell MC, Vranic M, et al. Insulin alone increases hypothalamo pituitary adrenal activity and diabetes lowers peak stress responses. Endocrinology 2005; 146(3): 1382-90.
Gawad FAA, Zaki SM, El-Shaarawy EZ, Radwan RA, Aboul-Hoda BE. Effect of Restraint Stress on the Adrenal Cortex of Prepubertal Male Albino Rat: A Histological and Immunohistochemical Study. Med. J. Cairo University 2016; 84(2): 257-62.
Piatti PM, Monti LD, Valsecchi G, Magni F, Setola E. Long term oral L-arginine administration improves peripheral and hepatic insulin sensitivity in type-2 diabetic patients. Diabetes Care 2001; 24(5): 875-80.
Chattopadhyay P, Shukla G , Wahi AK. Protective Effect of L-arginine Against Necrosis and Apoptosis Induced by Experimental Ischemic and Reperfusion in Rat Liver. The Saudi Journal of Gastroenterology 2009; 15(3): 156-62.
Quddus I, Kolachi GM, Qamar A, Ahmed R. Effects of L-arginine on fatty diet induced changes in adrenal cortex: A morphometric study. Medical Forum 2012; 23(2):40-44