• Afsheen Siddiqi Department of Pharmacology, Abbottabad
  • Saadia Shahzad Alam Department of Pharmacology, Federal Postgraduate Medical Institute, Shaikh Zayed Hospital, Lahore
  • Sajada Begum Allama Iqbal Medical College, Lahore
  • Zainab Nazneen Community Medicine, †Ophthalmology, Ayub Medical College Abbottabad
  • Bushra Aaqil


Background: Picrorhiza kurroa (Pk) is a traditional Ayurvedic herb famous as a potent hepatoprotective agent, only few studies are available on the nephroprotective activity of this herb. The objective of this pilot study was to determine the therapeutic effectiveness of Pk against nimesulide induced toxicity. Methods: This laboratory based experimental study was conducted on mice at National Institute of Health, Islamabad from Dec 2012 to Jan 2013. The mice were divided in to 4 groups. One group was given only PK while the other three groups were given nimesulide in a dosage of 750 mg/kg body weight for 3 days to induce nephrotoxicity and protective effect of Pk was noted by giving 250 mg/kg and 500 mg/kg pk for 14 days to the two of the nimesulide induced nephrotoxicity groups. Biochemical assessment of kidney was done by measuring serum urea & creatinine. Also histology was done to confirm the findings of biochemical assessment. Results: In our pilot study out of 20 mice, 19 mice survived. Only 1 mouse of nimesulide group died. Mean serum urea of nimesulide group was 60 mg/dl and was decreased to 23 mg/dl and 25 mg/dl by two doses of Pk. Mean creatinine in group 2 was 0.55 mg/dl and was decreased to 0.21 and 0.19 mg/dl by two doses of Pk. Conclusion: Our study shows that nimesulide is a potential nephrotoxic drug and its toxic effects on kidney can be minimized by using glycosidal extract of Pk.Keywords: Nimesulide, Picrorhiza kurroa Pk, nephrotoxicity


Schetza M, Dasta J, Goldsteinc S, Golperd T. Drug-induced acute kidney injury. Curr Opin Crit Care 2005;11(6):555–65.

Taber SS, Pasko DA. The epidemiology of drug-induced disorders: the kidney. Expert Opin Drug Saf 2008;7(6):679–90.

Conaghan PG. A turbulent decade for NSAIDs: update on current concepts of classification, epidemiology, comparative efficacy, and toxicity. Rheumatol Int 2012;32(6):1491–1502.

Rainsford KD. Nimesulide – a multifactorial approach to inflammation and pain: scientific and clinical consensus. Curr Med Res Opin 2006;22(6):1161–70.

Sharma V, Guleria R. Recent banned drugs in India: A quick update. IJPRD 2012; 4: 32-7.

Chhabra P, Paul R. Microcrystal test for detection of alkaloids of Datura fastuosa and glycosides of Nerium odorum and Calatropis gigantean. Malaysian J Forensic Sci 2011;2(1):50–6.

Chatterjee M, Sil PC. Protective role of Phyllanthus niruri against nimesulide induced hepatic damage. Indian J Clin Biochem 2007;22(1):109–16.

Chauhan S, Nath N, Tule V. Antidiabetic and antioxidant effects of Picrorhiza Kurrooa rhizome extracts in diabetic rats. Indian J Clin Biochem 2008;23(3):238–42.

Qadir MI, Tahir M, Lone KP, Munir B, Sami W. Protective role of Ginseng against gentamicin induced changes in kidney of albino mice. J Ayub Med Coll Abbottabad 2011;23(4):53–7.

Salah-Eldin O, Abd El-Azim SA, Eldeib KM, Barakat MM. Is lysosomal enzymes changes important in the pathogenesis of liver and kidney injury induced by short and long term administration of some NSAID' drugs in rats? Life Sci J 2012;9(4):1102–13.

Yamgar S, Sali L, Salkar R, Jain NK, Gadgoli CH. Studies on nephroprotective and nephrocurative activity of ethanolic extract of Picrorhiza kurroa royle and arogyawardhini bati in rats. Int J Pharm Tech 2010;2:472–89.