• Abdul Hannan
  • Tabish Humayun
  • Muhammad Barkaat Hussain
  • Muhammad Yasir
  • Sumayya Sikandar


Background: Cholera is a major public health problem in developing countries of the world.Bacterial resistance, lack of surveillance data and proper microbiological facilities are majorproblems regarding diagnosis of cholera. The spread of microbial drug resistance is a global publichealth challenge that results in increased illness and death rate. Newer antimicrobials or agents areurgently required to overcome this problem. This work was therefore done to investigate theantimicrobial potential of onion against thirty-three clinical isolates of Vibrio cholerae. Methods:The extract was prepared by reflux extraction method. Antibacterial screening of clinical isolatesof V. cholerae was done by agar well diffusion method. Agar dilution method was used to assessthe Minimum Inhibitory Concentration (MIC). Results: All tested strains of V. cholerae weresensitive to onion (Allium cepa) extracts of two types (purple and yellow). Purple type of extracthad MIC range of 19.2–21.6 mg/ml. The extract of yellow type onion had an MIC range of 66–68.4 mg/ml. Conclusion: The results indicated that onion (Allium cepa) has an inhibitory effect onV. cholerae. Keeping in view the anti-bacterial activity of this compound can be exploited as atherapeutic agent in an animal model. This finding is a positive point for further investigation ofthis herb of traditional medicine.Keywords: Vibrio cholerae, resistance, Allium cepa, antibacterial activity, extract


Hannan A, Raja M, Usman M, Absar M. Gastro mystery of

Mirpur Khas (gastro) resolved. J Ayub Med Coll Abbottabad


Hartley DM, Morris JG, Smith Dl. Hyperinfectivity: a critical

element in the ability of Vibrio cholerae to cause epidemics. J

PLoS Med 2006;3(1):e7.

J Ayub Med Coll Abbottabad 2010;22(2) 163

Rafi S, Qureshi AH, Saeed W, Ali A, Ahmadani M, khawajisa.

Changing epidemiology and sensitivity pattern of Vibrio cholera

at Rawalpindi. Pak J Med Sci 2004;20:357–60.

Scrascia M, Forcillo M, Maimone F, Pazzani C. Susceptibility to

rifaximin of Vibrio cholera strains from different geographical

areas. J Anti Chemo 2003;52:303–5.

Ali M, Thomson M, Afzal M. Garlic and onions: their effect on

eicosanoid metabolism and its clinical relevance. Prostaglandins

Leukot Essent Fatty Acids 2000;62:55–73.

Lampe JW. Health effects of vegetables and fruits: assessing

mechanisms of action in human experimental studies. Am J Clin

Nutr 1999;70:475–90.

Roychowdhury A, Pan A, Dutta D, Mukhopadhyay AK,

Ramamurthy T, Nandy RK, et al. Emergence of tetracyclineresistant Vibrio cholerae O1 serotypes Inaba, in Kolkata, India.

Jpn J Infect Dis 2008;61:128–9.

Azu NC, Reginald A, Okoro N, Kalu J. Antibacterial activity of

Allium cepa (onions) and Zingiber officinale (ginger) on

Staphylococcus aureus and Pseudomonas aeruginosa isolated

from high vaginal swab. Internet J Trop Med 2007;2:1540–2681.

Azu NC, Onyeagba RA. Antimicrobial properties of extracts of

Allium cepa (onions) and Zingiber officinale (ginger) on

Escherichia coli, Salmonella typhi and Bacillus subtilis. Internet J

Trop Med 2007;3:351–72.

Chehregani A, Azimishad F, Alizade HH. Study on antibacterial

effects of some Allium species from Hamedan-Iran. Int J Agri

Biol 2007;9:873–6.

Benkeblia N. Antimicrobial activity of essential oil extracts of

various onions (Allium cepa) and garlic (Allium sativum).

Lebensm Wiss u-technol 2004;37:263–8.

Irkin R, Korukluoglu M. Control of Aspergillus niger with garlic,

onion and leek extracts. Afr J Biotechnol 2007;6:384–7.

Mahesh B, Satish S. Antimicrobial activity of some important

medicinal plants against plant and human pathogens. World J

Agri Sci 2008;4:839–43.

Melvin JM, Jayochitra J, Vijayapriaya M. Antimicrobial activity

of some common spices against certain human pathogens. J Med

Plants Res 2009;3:1134–6.

Sharma A. Kumar P. In vitro screening of the antibacterial

activity and identification of bioactive compounds from plants

against selected Vibrio spp. Pathogens. Turk J Biol


Sharma A, Kumar P, Chaturvedi N. Vibriocidal activity of

certain medicinal plants used in Indian folklore medicine by

tribals of Mahakoshal region of central India. Indian J Pharmacol





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