EVALUATION OF TRIGLYCERIDES LEVELS IN SERUM AND TEARS OF OBESE AND NON-OBESE NORMAL ADULT HUMANS AND ITS EFFECTS ON PUPILLARY RESPONSE
DOI:
https://doi.org/10.55519/JAMC-01-9111Abstract
Background: Triglycerides are a type of storage fat present in serum of both normal and obese individuals. Triglycerides are normally present in human tears. The presence of qualitative fats in the tears affect the pupillary response to the external light stimulus which is measured through portable field dark adaptometer (PFDA) device. The current study was conducted to evaluate the levels of triglycerides in serum and tears of obese and non-obese humans and its relationship with pupillary response. Methods: This descriptive cross-sectional study was conducted at Institute of Basic Medical Sciences, Khyber Medical University, Peshawar. A total of 500 participants were recruited out of which only 50 qualified for the study as per inclusion criteria. Out of these 50, 25 (50%) were obese and 25 (50%) were non-obese. Serum triglycerides were measured by using Micro lab 300 biochemistry analyzer, while thin layer chromatography was used to detect triglycerides in tear samples. Results: Triglycerides were detected only in tears of obese individuals. No statistically significant difference was observed in the serum levels of triglycerides between the two groups (p=0.849). Conclusion: The presence of triglycerides in tears of obese adults caused a low pupillary response as compared to normal individuals.References
Chapman KD, Dyer JM, Mullen RT. Biogenesis and functions of lipid droplets in plants thematic review series: lipid droplet synthesis and metabolism: from yeast to man. J Lipid Res 2012;53(2):215–26.
Pol A, Gross SP, Parton RG. Biogenesis of the multifunctional lipid droplet: lipids, proteins, and sites. J Cell Biol 2014;204(5):635–46.
Sposito AC, Caramelli B, Fonseca FA, Bertolami MC, Afiune Neto A, Souza AD, et al. IV Brazilian Guideline for Dyslipidemia and Atherosclerosis prevention: Department of Atherosclerosis of Brazilian Society of Cardiology. Arq Bras Cardiol 2007;88(Suppl 1):2–19.
Mohammad MA, Sunehag AL, Haymond MW. De-novo synthesis of milk triglycerides in humans. Am J Physiol Endocrinol Metab 2014;306(7):E838–47.
Sarwar N, Danesh J, Eiriksdottir G, Sigurdsson G, Wareham N, Bingham S, et al. Triglycerides and the risk of coronary heart disease: 10 158 incident cases among 262 525 participants in 29 Western prospective studies. Circulation 2007;115(4):450–8.
Boullart A, De Graaf J, Stalenhoef A. Serum triglycerides and risk of cardiovascular disease. Biochim Biophys Acta 2012;1821(5):867–75.
Budoff M. Triglycerides and triglyceride-rich lipoproteins in the causal pathway of cardiovascular disease. Am J Cardiol 2016;118(1):138–45.
Brauninger GE, Shah DO, Kaufman HE. Direct physical demonstration of oily layer on tear film surface. Am J Ophthalmol 1972;73(1):132–4.
McCulley J, Shine W. A compositional based model for the tear film lipid layer. Trans Am Ophthalmol Soc 1997;95:79–88.
Rantamäki AH, Seppänen-Laakso T, Oresic M, Jauhiainen M, Holopainen JM. Human tear fluid lipidome: from composition to function. PLoS One 2011;6(5):e19553.
Kijlstra A, Kuizenga A. Analysis and function of the human tear proteins. Lacrimal Gland, Tear Film, and Dry Eye Syndromes: Springer, 1994; p.299–308.
Fleiszig SM, McNamara NA, Evans DJ. The tear film and defense against infection. Lacrimal Gland, Tear Film, and Dry Eye Syndromes 3: Springer, 2002; p.523–30.
Saville JT, Zhao Z, Willcox MD, Ariyavidana MA, Blanksby SJ, Mitchell TW. Identification of phospholipids in human meibum by nano-electrospray ionisation tandem mass spectrometry. Exp Eye Res 2011;92(3):238–40.
Khanal S, Millar TJ. Nanoscale phase dynamics of the normal tear film. Nanomedicine 2010;6(6):707–13.
Labrique AB, Palmer AC, Healy K, Mehra S, Sauer TC, West KP, et al. A novel device for assessing dark adaptation in field settings. BMC Ophthalmol 2015;15(1):74. Ref no 15&17 are same
Palmer AC, Healy K, Barffour MA, Siamusantu W, Chileshe J, Schulze KJ, et al. Provitamin A carotenoid–biofortified maize consumption increases pupillary responsiveness among Zambian children in a randomized controlled trial. J Nutr 2016;146(12):2551–8.
Labrique AB, Palmer AC, Healy K, Mehra S, Sauer TC, West KP, et al. A novel device for assessing dark adaptation in field settings. BMC Ophthalmol 2015;15(1):74. Ref no 15&17 are same
Nogaroto V, Rodrigues MR, Vicari MR, Almeida MC, Milleo FQ, Santos FA, et al. High postprandial triglycerides serum levels: is obesity a good predictor? An Acad Bras Ciênc 2015;87(1):437–45. Ref no 18&20 are same
Congdon NG, Dreyfuss ML, Christian P, Navitsky RC, Sanchez AM, Wu LS, et al. Responsiveness of dark-adaptation threshold to vitamin A and β-carotene supplementation in pregnant and lactating women in Nepal. Am J Clin Nutr 2000;72(4):1004–9.
Nogaroto V, Rodrigues MR, Vicari MR, Almeida MC, Milleo FQ, Santos FA, et al. High postprandial triglycerides serum levels: Is obesity a good predictor? An Acad Bras Ciênc 2015;87(1):437–45. Ref no 18&20 are same
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