HEPATITIS B, C AND HIV EXPOSURE ON NAT AND CLIA BLOOD EXAMINATION METHODS AT REGIONAL BLOOD CENTRE ABBOTTABAD
DOI:
https://doi.org/10.55519/JAMC-02-11600Keywords:
Transfusion Transmitted Infection, NAT, CLIAAbstract
Background: Blood transfusion is a lifesaving method in clinical emergencies. Despite various preventive measures, the spread of Hepatitis B, C and HIV remains a big issue in Pakistan. This study was done to describe transfusion transmitted diseases using NAT and CLIA techniques, on exposure to these viruses. Method: This study was conducted from 1st April to 25th August 2022. A descriptive study was done along with univariate analysis. The data was obtained from the regional blood centre in Abbottabad and it consists of reactive and non-reactive cases of NAT and CLIA in the sample size of 6233 donors. Data was collected from donors, and selected according to predefined criteria. Result: In 6233 samples, 53 were reactive for either Hepatitis B, C or HIV. Forty-seven were reactive with both CLIA and NAT. 6 were reactive with NAT only and 6107 were non-reactive. Conclusion: NAT yield detected in this study is 0.096%. (1:1039 donations). It implies that NAT should be the preferred method for screening in blood banks.References
Zaheer HA, Waheed U. Blood safety system reforms in Pakistan. Blood Transfus 2014;12(4):452–7.
Qureshi H, Bile KM, Jooma R, Alam SE, Afrid HU. Prevalence of hepatitis B and C viral infections in Pakistan: findings of a national survey appealing for effective prevention and control measures. East Mediterr Health J 2010;16(Supp):15–23.
Al Kanaani Z, Mahmud S, Kouyoumjian SP, Abu-Raddad LJ. The epidemiology of hepatitis C virus in Pakistan: systematic review and meta-analyses. R Soc Open Sci 2018;5(4):180257.
National blood policy and strategic framework, 2014-20. [Internet]. Safe Blood Transfusion Programme, Ministry of National Health Services, Regulation and Coordination Government of Pakistan 2014-20. [cited 2022 Oct 18]. Available from: https://pbta.punjab.gov.pk/system/files/Policies.pdf
Mathur A, Dontula S, Jagannathan L. A study of centralized individual donor nucleic acid testing for transfusion transmitted infections to improve blood safety in Karnataka, India. Glob J Transfus Med 2017;2(1):24–8.
Roth WK, Busch MP, Schuller A, Ismay S, Cheng A, Seed CR, et al. International survey on NAT testing of blood donations: expanding implementation and yield from 1999 to 2009. Vox Sang 2012;102(1):82–90.
Yang MH, Li L, Hung YS, Hung CS, Allain JP, Lin KS, et al. The efficacy of individual‐donation and minipool testing to detect low‐level hepatitis B virus DNA in Taiwan. Transfusion 2010;50(1):65–74.
Palla P, Vatteroni ML, Vacri L, Maggi F, Baicchi U. HIV‐1 NAT minipool during the pre‐seroconversion window period: detection of a repeat blood donor. Vox Sang 2006;90(1):59–62.
Jackson BR, Busch MP, Stramer SL, AuBuchon JP. The cost-effectiveness of NAT for HIV, HCV, and HBV in whole-blood donations. Transfusion 2003;43(6):721–9.
Makroo RN, Choudhury N, Jagannathan L, Parihar-Malhotra M, Raina V, Chaudhary RK, et al. Multicenter evaluation of individual donor nucleic acid testing (NAT) for simultaneous detection of human immunodeficiency virus -1 & hepatitis B & C viruses in Indian blood donors. Indian J Med Res 2008;127(2):140.
Roth WK, Weber M, Buhr S, Drosten C, Weichert W, Sireis W, et al. Yield of HCV and HIV-1 NAT after screening of 3.6 million blood donations in central Europe. Transfusion 2002;42(7):862–8.
Zou S, Dorsey KA, Notari EP, Foster GA, Krysztof DE, Musavi F, et al. Prevalence, incidence, and residual risk of human immunodeficiency virus and hepatitis C virus infections among United States blood donors since the introduction of nucleic acid testing. Transfusion 2010;50(7):1495–504.
Jain R, Aggarwal P, Gupta GN. Need for nucleic acid testing in countries with high prevalence of transfusion transmitted infections. ISRN Hematol 2012;2012:718671.
El Ekiaby M, Lelie N, Allain JP. Nucleic acid testing (NAT) in high prevalence-low resource setting. Biologicals 2010;38(1):59–64.
Weusten J, Vermeulen M, van Drimmelen H, Lelie N. Refinement of a viral transmission risk model for blood donations in seroconversion window phase screened by nucleic acid testing in different pool sizes and repeat test algorithms. Transfusion 2011;51(1):203–15.
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