• Abdul Hannan
  • Saadia Chaudhary
  • Sidrah Saleem
  • Atika Qayyum
  • Muhammad Usman Arshad


Background: To compare Bactec MGIT 960 with LJ media in terms of time taken for the initialisolation of mycobacteria. Methods: A total of 100 AFB (acid fast bacillus) positive sputumsamples were processed and inoculated in both the Lowenstein Jensen (LJ) media andmycobacterium growth indicator tube (MGIT) tubes. Results: Of the 100 samples, positive growthwas obtained from all the samples on both the MGIT and LJ media. In MGIT 53% samples grewin 4 days, 30% in 5 days and 17% in 6 days (Mean=4.6 days) while on LJ media, 44% grew in 30days, 20% in 35 days and 36% in 44 days (Mean=37 days). Significant difference was observedbetween two systems with a p-value of less than 0.05. Conclusion: Bactec MGIT 960 is a muchfaster and efficient system for the initial isolation of mycobacteria.Keywords: Mycobacteria, MGIT, LJ


Khosravi AD, Dezfulian A, Alavi SM. Detection of Isoniazid

and Rifampin resistant Mycobacterium tuberculosis isolated

from tuberculosis patients using conventional method and

PCR. Pak J Med Sci 2006;22:47–50.

Rasul S, Shabbir I, Lqbal R, Masood-ul-Haq, Khan SU,

Saeed S, et al. Trends in Multi Drug Resistant Tuberculosis.

Pak J Chest Med 2001;7:21–8.

Dhingra V, Nishi A, Rajpal S. Validity and Reliability of

sputum smear examination as diagnostic and screening test

for Tuberculosis. Ind J Astma Immunol. 2003;17:67–69.

Watterson S, Drobniewski F A. Modern laboratory diagnosis

of mycobacterial Infections. J Clin Pathol. 2000;53:727–32.

Crofton J, Chaulet P, Maher D. Guidelines for the

management of drug resistant tuberculosis. WHO/TB/96.210

Rev1. Geneva: World Health Organization; 1997.

Tenover FC, Crawford JT, Huebner RE, Geiter LJ,

Horsburgh CR Jr, Good RC. The resurgence of tuberculosis:

is your laboratory ready? J Clin Microbiol 1993;31:767–70.

Tortoli E, Cichero P, Piersimoni C, Simonetti MT, Gesu G,

Nista D. Use of BACTEC MGIT 960 for recovery of

mycobacteria from clinical specimens: multicentre study. J

Clin Microbiol. 1999;37:3578–82.

J Ayub Med Coll Abbottabad 2008;20(4)

http://www.ayubmed.edu.pk/JAMC/PAST/20-4/Hannan.pdf 69

Idigoras P, Beristain X, Iturzaeta A, Vicente D, PerezTrallero E. Comparison of the automated nonradiometric

Bactec MGIT 960 system and Lo¨wenstein-Jensen, Coletsos,

and Middlebrook 7H11 solid media for recovery of

mycobacteria. Eur J Clin Microb Infect Dis. 2000;19:350–54.

Telles MAS, Bori A, Amorim ABR, Cruz AF, Pini MIT,

Sato DN. Rapid detection of multidrug-resistant

Mycobacterium tuberculosis using the growth indicator tube

(MGIT) system. Braz J Med Biol. Res. 2002;35:1127–31.

Badak FZ, Kiska DL, Setterquist S, Hartley C, O’Connell

MA, Hopfer RL. Comparison of mycobacteria growth

indicator tube with BACTEC 460 for detection and recovery

of mycobacteria from clinical specimens. J Clin Microbiol


Pfyffer GE, Welscher HM, Kissling P, et al. Comparison of

the Mycobacteria Growth Indicator Tube (MGIT) with

radiometric and solid culture for recovery of acid-fast bacilli.

J Clin Microbiol. 1997;35:364–68.



Most read articles by the same author(s)

1 2 > >>