EFFICACY OF AMIKACIN AND CIPROFLOXACIN AGAINST CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS
AbstractBackground: Tuberculosis was a leading cause of death at the turn of the 20th century and continues tobe one of the medical scourges of mankind. Before the availability of antimicrobial drugs thecornerstone of treatment was rest in the open air in sanatoria. The major breakthrough in treatment oftuberculosis came with the discovery of Streptomycin. Later, INH, Ethambutol, Pyrazinamide,Rifampicin were added to the arsenal. Objective of this study was to determine the sensitivity of clinicalisolates of Mycobacterium tuberculosis against two second-line anti-tuberculosis drugs, Amikacin andCiprofloxacin. Methods: This cross-sectional study was conducted at Department of Microbiology,Armed Forces Institute of Pathology (AFIP) Rawalpindi. All routine clinical samples received for acidfast bacilli (AFB) in the Department of Microbiology, AFIP, Rawalpindi were processed by modifiedPetroff’s technique and inoculated on Lowenstein Jensen (LJ) medium and Bactec 460 Mycobacteriumtuberculosis culture system. After identification of M. tuberculosis sensitivity was performed againstfirst-line anti-tuberculosis drugs. Then susceptibility of M. tuberculosis isolates against Amikacin andCiprofloxacin was performed on LJ medium. H37Rv was used as control strain. Results: Results wereinterpreted using resistance ratio method. Out of 100 M. tuberculosis isolates, 98% were sensitive toAmikacin and 97% to Ciprofloxacin. Conclusion: Amikacin and Ciprofloxacin are very effective 2ndline anti-tuberculosis drugs against tuberculosis isolates in our set-up.Keywords: Tuberculosis, MDR-tuberculosis, susceptibility, second-line anti-tuberculosis drugs
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