DIAGNOSTIC SIGNIFICANCE OF ADENOSINE DEAMINASE IN PLEURAL TUBERCULOSIS
AbstractBackground: Tuberculosis (TB) is a major cause of pleural effusion, which in TB usually haslymphocytic and exudative characteristics. Analysis of adenosine deaminase (ADA) activity is avery useful diagnostic approach to achieve a more rapid and precise diagnosis in cases of PleuralTB (pTB). Methods: Fifty male and fifty female patients presenting with tuberculous pleuraleffusion was included in the study. The patients were taken from the medical ward of Sir GangaRam Hospital between September 2001 and September 2002. Activity of Adenosine Deaminase(ADA) was estimated by the technique of Sodium dodecyl sulphate electrophoresis (SDS-EF)using 10% polyacrylamide gel. Results: Mean age of males was 45.72±19.22 years and of femalewas 43.74±16.09 years. Mean protein level was 3.39±0.24 g/dl in males, and it was 3.02±0.26 g/dlin females. Mean specific gravity both in males and females was 1.020±0.01. The results show anincreased level of enzyme ADA in patients as compared to normal subjects. Conclusion:Estimation of ADA activity may provide basis for rapid and efficient diagnosis of pleural TB indifferent clinical settings. However study should be extended to larger number of patients to reacha better conclusion.Keywords: Adenosine deaminase, Pleural TB, Electrophoresis
Harries AD. Tuberculosis and human immunodeficiency
virus infection in developing countries. Lancet
Agrons GA, Markowitz RI, Kramer SS. Pulmonary tuberculosis
in children. Semin Roentgenol 1993;28(2):158–72.
Aggarwal AN, Gupta D, Jindal SK. Diagnosis of tuberculous
pleural effusion. Indian J Chest Dis Allied Sci
Sahn, SA. The diagnostic value of pleural fluid analysis.
Seminars in Respir and Crit Care Med 1995;16(4):269–78.
Tarn AC and Lapworth R. Biochemical analysis of pleural
fluid: What should we measure? Annals of Clinical
Kent PT, Kubica GP. Public health mycobacteriology: a
guide for level III laboratory. Atlanta: US Dept. of Health and
Human Services, Public Health Service, Centers for Disease
Control (Atlanta, Ga). 1985.
Gopi A, Madhavan SM, Sharma SK, Sahn SA. Diagnosis and
treatment of tuberculous pleural effusion in 2006. Chest
Dinnes J, Deeks J, Kunst H, Gibson A, Cummins E, Waugh
N, et al. A systematic review of rapid diagnostic tests for the
detection of tuberculosis infection. Health Technol Assess
J Ayub Med Coll Abbottabad 2009;21(1)
Lima DM, Colares JK, da Fonseca BA. Combined use of the
polymerase chain reaction and detection of adenosine
deaminase activity on pleural fluid improves the rate of
diagnosis of pleural tuberculosis. Chest 2004;125(6):2366.
Poyraz B, Kaya A, Oktem A. Diagnostic significance of
gamma-interferon in tuberculous pleurisy. Tuberk Toraks
Villegas MV, Labrada LA, Saravia NG. Evaluation of
polymerase chain reaction, adenosine deaminase, and
interferon-gamma in pleural fluid for the differential diagnosis
of pleural tuberculosis. Chest 2000;118(5):1355–64.
He Q, Xie CM, Tan SY, Zhang KX, Deng XH, Zhu PB et al.
[A study on the model of tuberculous pleurisy and
intrapleural inflammatory and immunological responses in
rats.] Zhonghua Jie He He Hu Xi Za Zhi 2005;28(2):117–21.
Venkatesh J, Oommen A. A molecular weight study of
adenosine deaminase. Indian J Biochem Biophys
Wiginton DA, Coleman MS, Hutton JJ. Purification,
characterization and radioimmunoassay of adenosine
deaminase from human leukaemic granulocytes. Biochem J
Ferrer Sancho J. Pleural tuberculosis: incidence,
pathogenesis, diagnosis, and treatment. Curr Opin Pulm Med